1b7e
From Proteopedia
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'''TRANSPOSASE INHIBITOR''' | '''TRANSPOSASE INHIBITOR''' | ||
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[[Category: Rayment, I.]] | [[Category: Rayment, I.]] | ||
[[Category: Reznikoff, W S.]] | [[Category: Reznikoff, W S.]] | ||
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Polynucleotidyl transferase]] |
| - | [[Category: | + | [[Category: Transposase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:09:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:09, 2 May 2008
TRANSPOSASE INHIBITOR
Overview
Transposon Tn5 employs a unique means of self-regulation by expressing a truncated version of the transposase enzyme that acts as an inhibitor. The inhibitor protein differs from the full-length transposase only by the absence of the first 55 N-terminal amino acid residues. It contains the catalytic active site of transposase and a C-terminal domain involved in protein-protein interactions. The three-dimensional structure of Tn5 inhibitor determined to 2.9-A resolution is reported here. A portion of the protein fold of the catalytic core domain is similar to the folds of human immunodeficiency virus-1 integrase, avian sarcoma virus integrase, and bacteriophage Mu transposase. The Tn5 inhibitor contains an insertion that extends the beta-sheet of the catalytic core from 5 to 9 strands. All three of the conserved residues that make up the "DDE" motif of the active site are visible in the structure. An arginine residue that is strictly conserved among the IS4 family of bacterial transposases is present at the center of the active site, suggesting a catalytic motif of "DDRE." A novel C-terminal domain forms a dimer interface across a crystallographic 2-fold axis. Although this dimer represents the structure of the inhibited complex, it provides insight into the structure of the synaptic complex.
About this Structure
1B7E is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution., Davies DR, Mahnke Braam L, Reznikoff WS, Rayment I, J Biol Chem. 1999 Apr 23;274(17):11904-13. PMID:10207011 Page seeded by OCA on Fri May 2 11:09:54 2008
