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6kdv

From Proteopedia

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Current revision

Crystal structure of TtCas1-DNA complex

Structural highlights

6kdv is a 8 chain structure with sequence from Thet8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	cas1-2, TTHB193 (THET8)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAS1B_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.

Publication Abstract from PubMed

Cas1 is a key component of the CRISPR adaptation complex, which captures and integrates foreign DNA into the CRISPR array, resulting in the generation of new spacers. We have determined crystal structures of Thermus thermophilus Cas1 involved in new spacer acquisition both in complex with branched DNA and in the free state. Cas1 forms an asymmetric dimer without DNA. Conversely, two asymmetrical dimers bound to two branched DNAs result in the formation of a DNA-mediated tetramer, dimer of structurally asymmetrical dimers, in which the two subunits markedly present different conformations. In the DNA binding complex, the N-terminal domain adopts different orientations with respect to the C-terminal domain in the two monomers that form the dimer. Substrate binding triggers a conformational change in the loop 164-177 segment. This loop is also involved in the 3' fork arm and 5' fork arm strand recognition in monomer A and B, respectively. This study provides important insights into the molecular mechanism of new spacer adaptation.

Crystal structure of Cas1 in complex with branched DNA.,Yang J, Li J, Wang J, Sheng G, Wang M, Zhao H, Yang Y, Wang Y Sci China Life Sci. 2019 Nov 28. pii: 10.1007/s11427-019-9827-x. doi:, 10.1007/s11427-019-9827-x. PMID:31792780^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yang J, Li J, Wang J, Sheng G, Wang M, Zhao H, Yang Y, Wang Y. Crystal structure of Cas1 in complex with branched DNA. Sci China Life Sci. 2019 Nov 28. pii: 10.1007/s11427-019-9827-x. doi:, 10.1007/s11427-019-9827-x. PMID:31792780 doi:http://dx.doi.org/10.1007/s11427-019-9827-x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kdv"

@@ Line 3: / Line 3: @@
 <StructureSection load='6kdv' size='340' side='right'caption='[[6kdv]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kdv]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KDV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KDV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kdv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KDV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KDV FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kdv OCA], [http://pdbe.org/6kdv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kdv RCSB], [http://www.ebi.ac.uk/pdbsum/6kdv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kdv ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas1-2, TTHB193 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kdv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kdv OCA], [http://pdbe.org/6kdv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kdv RCSB], [http://www.ebi.ac.uk/pdbsum/6kdv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kdv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
+[[Category: Thet8]]
 [[Category: Li, J Z]]
 [[Category: Wang, J Y]]

6kdv

From Proteopedia

Current revision

Crystal structure of TtCas1-DNA complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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