2l4g

From Proteopedia

(Difference between revisions)

Revision as of 06:59, 6 May 2020

Influenza Haemagglutinin fusion peptide mutant G13A

Structural highlights

2l4g is a 1 chain structure with sequence from I000x. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2jrd, 1ibn, 1xop, 1xoo
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q9DHG0_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).[SAAS:SAAS00204388]

Publication Abstract from PubMed

Our previous studies showed that an angled boomerang-shaped structure of the influenza hemagglutinin (HA) fusion domain is critical for virus entry into host cells by membrane fusion. Because the acute angle of approximately 105 degrees of the wild-type fusion domain promotes efficient non-leaky membrane fusion, we asked whether different angles would still support fusion and thus facilitate virus entry. Here, we show that the G13A fusion domain mutant produces a new leaky fusion phenotype. The mutant fusion domain structure was solved by NMR spectroscopy in a lipid environment at fusion pH. The mutant adopted a boomerang structure similar to that of wild type but with a shallower kink angle of approximately 150 degrees . G13A perturbed the structure of model membranes to a lesser degree than wild type but to a greater degree than non-fusogenic fusion domain mutants. The strength of G13A binding to lipid bilayers was also intermediate between that of wild type and non-fusogenic mutants. These membrane interactions provide a clear link between structure and function of influenza fusion domains: an acute angle is required to promote clean non-leaky fusion suitable for virus entry presumably by interaction of the fusion domain with the transmembrane domain deep in the lipid bilayer. A shallower angle perturbs the bilayer of the target membrane so that it becomes leaky and unable to form a clean fusion pore. Mutants with no fixed boomerang angle interacted with bilayers weakly and did not promote any fusion or membrane perturbation.

Shallow boomerang-shaped influenza hemagglutinin G13A mutant structure promotes leaky membrane fusion.,Lai AL, Tamm LK J Biol Chem. 2010 Nov 26;285(48):37467-75. Epub 2010 Sep 8. PMID:20826788^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lai AL, Tamm LK. Shallow boomerang-shaped influenza hemagglutinin G13A mutant structure promotes leaky membrane fusion. J Biol Chem. 2010 Nov 26;285(48):37467-75. Epub 2010 Sep 8. PMID:20826788 doi:10.1074/jbc.M110.153700

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l4g"

@@ Line 1: / Line 1: @@
 ==Influenza Haemagglutinin fusion peptide mutant G13A==
-<StructureSection load='2l4g' size='340' side='right' caption='[[2l4g]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2l4g' size='340' side='right'caption='[[2l4g]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2l4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/I000x I000x]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L4G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2l4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/I000x I000x]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2L4G FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jrd|2jrd]], [[1ibn|1ibn]], [[1xop|1xop]], [[1xoo|1xoo]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4g OCA], [http://pdbe.org/2l4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l4g RCSB], [http://www.ebi.ac.uk/pdbsum/2l4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4g ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2l4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4g OCA], [http://pdbe.org/2l4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l4g RCSB], [http://www.ebi.ac.uk/pdbsum/2l4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: I000x]]
+[[Category: Large Structures]]
 [[Category: Lai, A L]]
 [[Category: Tamm, L K]]
 [[Category: Fusion peptide]]
 [[Category: Viral protein]]

2l4g

From Proteopedia

Revision as of 06:59, 6 May 2020

Influenza Haemagglutinin fusion peptide mutant G13A

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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