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2l72

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Revision as of 06:59, 6 May 2020

Solution structure and dynamics of ADF from Toxoplasma gondii (TgADF)

Structural highlights

2l72 is a 1 chain structure with sequence from Toxgo. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Toxoplasma gondii ADF (TgADF) belongs to a functional subtype characterized by strong G-actin sequestering activity and low F-actin severing activity. Among the characterized ADF/cofilin proteins, TgADF has the shortest length and is missing a C-terminal helix implicated in F-actin binding. In order to understand its characteristic properties, we have determined the solution structure of TgADF and studied its backbone dynamics from (15)N-relaxation measurements. TgADF has conserved ADF/cofilin fold consisting of a central mixed beta-sheet comprised of six beta-strands that are partially surrounded by three alpha-helices and a C-terminal helical turn. The high G-actin sequestering activity of TgADF relies on highly structurally and dynamically optimized interactions between G-actin and G-actin binding surface of TgADF. The equilibrium dissociation constant for TgADF and rabbit muscle G-actin was 23.81nM, as measured by ITC, which reflects very strong affinity of TgADF and G-actin interactions. The F-actin binding site of TgADF is partially formed, with a shortened F-loop that does not project out of the ellipsoid structure and a C-terminal helical turn in place of the C-terminal helix alpha4. Yet, it is more rigid than the F-actin binding site of Leishmania donovani cofilin. Experimental observations and structural features do not support the interaction of PIP2 with TgADF, and PIP2 does not affect the interaction of TgADF with G-actin. Overall, this study suggests that conformational flexibility of G-actin binding sites enhances the affinity of TgADF for G-actin, while conformational rigidity of F-actin binding sites of conventional ADF/cofilins is necessary for stable binding to F-actin.

Solution structure and dynamics of ADF from Toxoplasma gondii.,Yadav R, Pathak PP, Shukla VK, Jain A, Srivastava S, Tripathi S, Krishna Pulavarti SV, Mehta S, David Sibley L, Arora A J Struct Biol. 2011 Jul 26. PMID:21820516^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yadav R, Pathak PP, Shukla VK, Jain A, Srivastava S, Tripathi S, Krishna Pulavarti SV, Mehta S, David Sibley L, Arora A. Solution structure and dynamics of ADF from Toxoplasma gondii. J Struct Biol. 2011 Jul 26. PMID:21820516 doi:10.1016/j.jsb.2011.07.011

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l72"

@@ Line 1: / Line 1: @@
 ==Solution structure and dynamics of ADF from Toxoplasma gondii (TgADF)==
-<StructureSection load='2l72' size='340' side='right' caption='[[2l72]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2l72' size='340' side='right'caption='[[2l72]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2l72]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L72 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2l72]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L72 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2L72 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l72 OCA], [http://pdbe.org/2l72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l72 RCSB], [http://www.ebi.ac.uk/pdbsum/2l72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l72 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2l72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l72 OCA], [http://pdbe.org/2l72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l72 RCSB], [http://www.ebi.ac.uk/pdbsum/2l72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l72 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 19: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Toxgo]]
 [[Category: Arora, A]]

2l72

From Proteopedia

Revision as of 06:59, 6 May 2020

Solution structure and dynamics of ADF from Toxoplasma gondii (TgADF)

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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