4yr6

Publication Abstract from PubMed

Ectodomain shedding of glycoprotein (GP) Ibalpha is thought to mediate the clearance of activated, aged or damaged platelets. A monoclonal antibody, 5G6, has been developed recently to specifically bind to the GPIbalpha shedding cleavage site and to inhibit its shedding. However, the molecular mechanism underlying antigen recognition and inhibitory specificity is not clear. To elucidate the structural basis for 5G6 binding to GPIbalpha, we determined the crystal structure of 5G6 Fab fragment in complex with its epitope peptide KL10 (GPIbalpha residues 461-470, KLRGVLQGHL), to 2.4-A resolution. Key residues in both 5G6 and KL10 were mutated to validate their effects in antibody binding by using isothermal titration calorimetry. The 5G6 Fab-KL10 peptide complex structure confirmed the direct association of 5G6 with its target GPIbalpha residues and elucidated the molecular basis underlying its binding specificity and high affinity. The similar binding properties of 5G6 Fab fragment to GPIbalpha on human platelets as those to KL10 suggests that such an interaction may not be affected by the plasma membrane or nearby GPIbbeta. This structural information may facilitate further antibody optimization and humanization.

Structural basis for the specific inhibition of glycoprotein Ibalpha shedding by an inhibitory antibody.,Tao Y, Zhang X, Liang X, Zang J, Mo X, Li R Sci Rep. 2016 Apr 22;6:24789. doi: 10.1038/srep24789. PMID:27102061^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.