6tc5
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Phytochromobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB== | ==Phytochromobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB== | ||
| - | <StructureSection load='6tc5' size='340' side='right'caption='[[6tc5]]' scene=''> | + | <StructureSection load='6tc5' size='340' side='right'caption='[[6tc5]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TC5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TC5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tc5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Andropogon_sorghum Andropogon sorghum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TC5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TC5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tc5 OCA], [http://pdbe.org/6tc5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tc5 RCSB], [http://www.ebi.ac.uk/pdbsum/6tc5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tc5 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=O6E:3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic+acid'>O6E</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHYB, SORBI_3001G394400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4558 Andropogon sorghum])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tc5 OCA], [http://pdbe.org/6tc5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tc5 RCSB], [http://www.ebi.ac.uk/pdbsum/6tc5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tc5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/Q6S527_SORBI Q6S527_SORBI]] Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region.[PIRNR:PIRNR000084] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plant phytochromes are red/far-red photochromic photoreceptors that act as master regulators of development, controlling the expression of thousands of genes. Here, we describe the crystal structures of four plant phytochrome sensory modules, three at about 2 A resolution or better, including the first of an A-type phytochrome. Together with extensive spectral data, these structures provide detailed insight into the structure and function of plant phytochromes. In the Pr state, the substitution of phycocyanobilin and phytochromobilin cofactors has no structural effect, nor does the amino-terminal extension play a significant functional role. Our data suggest that the chromophore propionates and especially the phytochrome-specific domain tongue act differently in plant and prokaryotic phytochromes. We find that the photoproduct in period-ARNT-single-minded (PAS)-cGMP-specific phosphodiesterase-adenylyl cyclase-FhlA (GAF) bidomains might represent a novel intermediate between MetaRc and Pfr. We also discuss the possible role of a likely nuclear localization signal specific to and conserved in the phytochrome A lineage. | ||
| + | |||
| + | Structural insights into photoactivation and signalling in plant phytochromes.,Nagano S, Guan K, Shenkutie SM, Feiler C, Weiss M, Kraskov A, Buhrke D, Hildebrandt P, Hughes J Nat Plants. 2020 May 4. pii: 10.1038/s41477-020-0638-y. doi:, 10.1038/s41477-020-0638-y. PMID:32366982<ref>PMID:32366982</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6tc5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Andropogon sorghum]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Guan K]] | + | [[Category: Guan, K]] |
| - | [[Category: Hughes | + | [[Category: Hughes, J E]] |
| - | [[Category: Nagano S]] | + | [[Category: Nagano, S]] |
| - | [[Category: Shenkutie | + | [[Category: Shenkutie, S M]] |
| + | [[Category: Phytochrome]] | ||
| + | [[Category: Plant protein]] | ||
Revision as of 05:55, 13 May 2020
Phytochromobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB
| |||||||||||
