5g48

From Proteopedia

(Difference between revisions)

Revision as of 06:38, 13 May 2020

H.pylori Beta clamp in complex with Diflunisal

Structural highlights

5g48 is a 2 chain structure with sequence from Helicobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DPO3B_HELPY] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA (By similarity).

Publication Abstract from PubMed

The beta-clamp is the processivity-promoting factor for most of the enzymes in prokaryotic DNA replication; hence, it is a crucial drug target. In the present study, we investigated the beta-clamp from Helicobacter pylori, aiming to seek potential drug molecules against this gastric-cancer-causing bacterium. An in silico screening of Food and Drug Administration (FDA) approved drugs against the H. pylori beta-clamp, followed by its in vitro inhibition using a surface competition approach, yielded the drug diflunisal as a positive initial hit. Diflunisal inhibits the growth of H. pylori in the micromolar range. We determined the structure of diflunisal in complex with the beta-clamp to show that the drug binds at subsite I, which is a protein-protein interaction site. Successful identification of FDA-approved molecules against H. pylori may lead to better and faster drug development.

Targeting the beta-clamp in Helicobacter pylori with FDA-approved drugs reveals micromolar inhibition by diflunisal.,Pandey P, Verma V, Gautam G, Kumari N, Dhar SK, Gourinath S FEBS Lett. 2017 Aug;591(15):2311-2322. doi: 10.1002/1873-3468.12734. Epub 2017, Jul 26. PMID:28656718^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pandey P, Verma V, Gautam G, Kumari N, Dhar SK, Gourinath S. Targeting the beta-clamp in Helicobacter pylori with FDA-approved drugs reveals micromolar inhibition by diflunisal. FEBS Lett. 2017 Aug;591(15):2311-2322. doi: 10.1002/1873-3468.12734. Epub 2017, Jul 26. PMID:28656718 doi:http://dx.doi.org/10.1002/1873-3468.12734

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5g48"

@@ Line 1: / Line 1: @@
 ==H.pylori Beta clamp in complex with Diflunisal==
-<StructureSection load='5g48' size='340' side='right' caption='[[5g48]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
+<StructureSection load='5g48' size='340' side='right'caption='[[5g48]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5g48]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G48 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5g48]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G48 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G48 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1FL:5-(2,4-DIFLUOROPHENYL)-2-HYDROXY-BENZOIC+ACID'>1FL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1FL:5-(2,4-DIFLUOROPHENYL)-2-HYDROXY-BENZOIC+ACID'>1FL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g48 OCA], [http://pdbe.org/5g48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g48 RCSB], [http://www.ebi.ac.uk/pdbsum/5g48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g48 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g48 OCA], [http://pdbe.org/5g48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g48 RCSB], [http://www.ebi.ac.uk/pdbsum/5g48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g48 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 5g48" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
 == References ==
 <references/>
@@ Line 23: / Line 26: @@
 </StructureSection>
 [[Category: Helicobacter pylori]]
+[[Category: Large Structures]]
 [[Category: Gourinath, S]]
 [[Category: Pandey, P]]

5g48

From Proteopedia

Revision as of 06:38, 13 May 2020

H.pylori Beta clamp in complex with Diflunisal

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox