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6s20

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Current revision

Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33336S-sulf)

Structural highlights

6s20 is a 1 chain structure with sequence from Bactn. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Gene:	BT_3333 (BACTN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GALSF_BACTN] Exosulfatase involved in the degradation of the glycosaminoglycans (GAGs) chondroitin sulfate (CS) and dermatan sulfate (DS). Catalyzes the hydrolysis of the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units (PubMed:25002587). GAG-specific sulfatases play a key role in the persistence of the major human gut symbiont B.thetaiotaomicron in the host gastrointestinal tract (PubMed:25002587).^[1] ^[2]

Publication Abstract from PubMed

The human gut microbiota (HGM), which is critical to human health, utilises complex glycans as its major carbon source. Glycosaminoglycans represent an important, high priority, nutrient source for the HGM. Pathways for the metabolism of various glycosaminoglycan substrates remain ill-defined. Here we perform a biochemical, genetic and structural dissection of the genetic loci that orchestrates glycosaminoglycan metabolism in the organism Bacteroides thetaiotaomicron. Here, we report: the discovery of two previously unknown surface glycan binding proteins which facilitate glycosaminoglycan import into the periplasm; distinct kinetic and genetic specificities of various periplasmic lyases which dictate glycosaminoglycan metabolic pathways; understanding of endo sulfatase activity questioning the paradigm of how the 'sulfation problem' is handled by the HGM; and 3D crystal structures of the polysaccharide utilisation loci encoded sulfatases. Together with comparative genomic studies, our study fills major gaps in our knowledge of glycosaminoglycan metabolism by the HGM.

Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus.,Ndeh D, Basle A, Strahl H, Yates EA, McClurgg UL, Henrissat B, Terrapon N, Cartmell A Nat Commun. 2020 Jan 31;11(1):646. doi: 10.1038/s41467-020-14509-4. PMID:32005816^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ulmer JE, Vilen EM, Namburi RB, Benjdia A, Beneteau J, Malleron A, Bonnaffe D, Driguez PA, Descroix K, Lassalle G, Le Narvor C, Sandstrom C, Spillmann D, Berteau O. Characterization of glycosaminoglycan (GAG) sulfatases from the human gut symbiont Bacteroides thetaiotaomicron reveals the first GAG-specific bacterial endosulfatase. J Biol Chem. 2014 Aug 29;289(35):24289-303. doi: 10.1074/jbc.M114.573303. Epub, 2014 Jul 7. PMID:25002587 doi:http://dx.doi.org/10.1074/jbc.M114.573303
↑ Ulmer JE, Vilen EM, Namburi RB, Benjdia A, Beneteau J, Malleron A, Bonnaffe D, Driguez PA, Descroix K, Lassalle G, Le Narvor C, Sandstrom C, Spillmann D, Berteau O. Characterization of glycosaminoglycan (GAG) sulfatases from the human gut symbiont Bacteroides thetaiotaomicron reveals the first GAG-specific bacterial endosulfatase. J Biol Chem. 2014 Aug 29;289(35):24289-303. doi: 10.1074/jbc.M114.573303. Epub, 2014 Jul 7. PMID:25002587 doi:http://dx.doi.org/10.1074/jbc.M114.573303
↑ Ndeh D, Basle A, Strahl H, Yates EA, McClurgg UL, Henrissat B, Terrapon N, Cartmell A. Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus. Nat Commun. 2020 Jan 31;11(1):646. doi: 10.1038/s41467-020-14509-4. PMID:32005816 doi:http://dx.doi.org/10.1038/s41467-020-14509-4

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6s20"

@@ Line 1: / Line 1: @@
-==Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT3336S-sulf)==
+==Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33336S-sulf)==
 <StructureSection load='6s20' size='340' side='right'caption='[[6s20]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6s20]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactn Bactn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S20 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6s20]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactn Bactn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S20 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S20 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NG6:N-ACETYL-D-GALACTOSAMINE+6-SULFATE'>NG6</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NG6:N-ACETYL-D-GALACTOSAMINE+6-SULFATE'>NG6</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BT_3333 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226186 BACTN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s20 OCA], [http://pdbe.org/6s20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s20 RCSB], [http://www.ebi.ac.uk/pdbsum/6s20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s20 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s20 OCA], [http://pdbe.org/6s20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s20 RCSB], [http://www.ebi.ac.uk/pdbsum/6s20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s20 ProSAT]</span></td></tr>
 </table>
 == Function ==

6s20

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Current revision

Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33336S-sulf)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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