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5h1x

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Revision as of 06:49, 20 May 2020

Crystal Structure of rat Nup62 Coiled-coil motif

Structural highlights

5h1x is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NUP62_RAT] Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.

Publication Abstract from PubMed

The central transport channel of the vertebrate nuclear pore complex (NPC) consists of nucleoporins: Nup62, Nup54, and Nup58. The coiled-coil domains in alpha-helical regions of these nucleoporins are thought to be crucial for several protein-protein interactions in the NPC subcomplexes. In this study, we determined the crystal structure of the coiled-coil domain of rat Nup62 fragment (residues 362-425) to 2.4 A resolution. The crystal structure shows the conserved coiled-coil domain as a parallel three-helix bundle for the Nup62(362-425) fragment. On the basis of our size exclusion chromatography coupled to multiangle light scattering analysis and glutaraldehyde cross-linking experiments, we conclude that the Nup62(362-425) fragment displays dynamic behavior in solution and can also exist in either homodimeric or homotrimeric states. Our comparative analysis of the rat Nup62(362-425) homotrimeric structure with previously reported heterotrimeric structures [rat Nup62(362-425).Nup54(346-407) and Xenopus Nup62(358-485).Nup54(315-450).Nup58(283-406) complexes] demonstrates the structural basis for parallel triple-helix bundle formation for Nup62 with different partners. Moreover, we show that the coiled-coil domain of Nup62 is sufficient for interaction with the coiled-coil domain of rat Exo70, a protein in an exocyst complex. On the basis of these observations, we suggest the plausible chain replacement mechanism that yields to diverse protein assemblies with Nup62. In summary, the coiled-coil motif present in Nup62 imparts the ability to form a homotrimer and heterotrimers either with Nup54 or with Nup54-Nup58 within the NPCs as well as with Exo70 beyond the NPCs. These complexes of Nup62 suggest the crucial role of the coiled-coil motifs in providing plasticity to various modular assemblies.

The Nup62 Coiled-Coil Motif Provides Plasticity for Triple-Helix Bundle Formation.,Dewangan PS, Sonawane PJ, Chouksey AR, Chauhan R Biochemistry. 2017 Jun 6;56(22):2803-2811. doi: 10.1021/acs.biochem.6b01050. Epub, 2017 Apr 26. PMID:28406021^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dewangan PS, Sonawane PJ, Chouksey AR, Chauhan R. The Nup62 Coiled-Coil Motif Provides Plasticity for Triple-Helix Bundle Formation. Biochemistry. 2017 Jun 6;56(22):2803-2811. doi: 10.1021/acs.biochem.6b01050. Epub, 2017 Apr 26. PMID:28406021 doi:http://dx.doi.org/10.1021/acs.biochem.6b01050

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h1x"

Categories: Large Structures | Pravin, D | Coiled-coil | Structural protein

@@ Line 1: / Line 1: @@
 ==Crystal Structure of rat Nup62 Coiled-coil motif==
-<StructureSection load='5h1x' size='340' side='right' caption='[[5h1x]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
+<StructureSection load='5h1x' size='340' side='right'caption='[[5h1x]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H1X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H1X FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [http://pdbe.org/5h1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [http://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [http://pdbe.org/5h1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [http://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 17: @@
 </div>
 <div class="pdbe-citations 5h1x" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Pravin, D]]
 [[Category: Coiled-coil]]
 [[Category: Structural protein]]

5h1x

From Proteopedia

Revision as of 06:49, 20 May 2020

Crystal Structure of rat Nup62 Coiled-coil motif

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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