5h28

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Revision as of 06:50, 20 May 2020

Crystal structure of Osh1 ANK domain from Saccharomyces cerevisia

Structural highlights

5h28 is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5h2a, 5h2c, 5h2d
Gene:	SWH1, OSH1, YAR042W, YAR044W (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[OSH1_YEAST] Lipid-binding protein involved in maintenance of intracellular sterol distribution and homeostasis. Binds to phosphoinositides. May be involved in formation of PMN vesicles by altering the membrane lipid composition.^[1]

Publication Abstract from PubMed

Yeast Osh1 belongs to the oxysterol-binding protein (OSBP) family of proteins and contains multiple targeting modules optimized for lipid transport at the nucleus-vacuole junction (NVJ). The key determinants for NVJ targeting and the role of Osh1 at NVJs have remained elusive because of unknown lipid specificities. In this study, we determined the structures of the ankyrin repeat domain (ANK), and OSBP-related domain (ORD) of Osh1, in complex with Nvj1 and ergosterol, respectively. The Osh1 ANK forms a unique bi-lobed structure that recognizes a cytosolic helical segment of Nvj1. We discovered that Osh1 ORD binds ergosterol and phosphatidylinositol 4-phosphate PI(4)P in a competitive manner, suggesting counter-transport function of the two lipids. Ergosterol is bound to the hydrophobic pocket in a head-down orientation, and the structure of the PI(4)P-binding site in Osh1 is well conserved. Our results suggest that Osh1 performs non-vesicular transport of ergosterol and PI(4)P at the NVJ.

Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction.,Manik MK, Yang H, Tong J, Im YJ Structure. 2017 Apr 4;25(4):617-629.e3. doi: 10.1016/j.str.2017.02.010. Epub 2017, Mar 16. PMID:28319008^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Beh CT, Rine J. A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution. J Cell Sci. 2004 Jun 15;117(Pt 14):2983-96. Epub 2004 Jun 1. PMID:15173322 doi:http://dx.doi.org/10.1242/jcs.01157
↑ Manik MK, Yang H, Tong J, Im YJ. Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction. Structure. 2017 Apr 4;25(4):617-629.e3. doi: 10.1016/j.str.2017.02.010. Epub 2017, Mar 16. PMID:28319008 doi:http://dx.doi.org/10.1016/j.str.2017.02.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h28"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Osh1 ANK domain from Saccharomyces cerevisia==
-<StructureSection load='5h28' size='340' side='right' caption='[[5h28]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+<StructureSection load='5h28' size='340' side='right'caption='[[5h28]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5h28]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H28 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5h28]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H28 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H28 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h2a|5h2a]], [[5h2c|5h2c]], [[5h2d|5h2d]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h28 OCA], [http://pdbe.org/5h28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h28 RCSB], [http://www.ebi.ac.uk/pdbsum/5h28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h28 ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SWH1, OSH1, YAR042W, YAR044W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h28 OCA], [http://pdbe.org/5h28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h28 RCSB], [http://www.ebi.ac.uk/pdbsum/5h28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h28 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5h28" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Oxysterol-binding protein homolog|Oxysterol-binding protein homolog]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Im, Y J]]
 [[Category: Manik, M K]]

5h28

From Proteopedia

Revision as of 06:50, 20 May 2020

Crystal structure of Osh1 ANK domain from Saccharomyces cerevisia

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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