1be4

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[[Image:1be4.gif|left|200px]]
[[Image:1be4.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1be4 |SIZE=350|CAPTION= <scene name='initialview01'>1be4</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1be4", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=NUL:C-Gmp+Binding+Site'>NUL</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1be4| PDB=1be4 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1be4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1be4 OCA], [http://www.ebi.ac.uk/pdbsum/1be4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1be4 RCSB]</span>
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}}
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'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA'''
'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA'''
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[[Category: Ridge, K D.]]
[[Category: Ridge, K D.]]
[[Category: Tordova, M.]]
[[Category: Tordova, M.]]
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[[Category: phosphotransferase]]
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[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:23:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:59:09 2008''
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Revision as of 08:23, 2 May 2008

Image:1be4.gif

Template:STRUCTURE 1be4

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA


Overview

The biochemical and structural properties of bovine retinal nucleoside diphosphate kinase were investigated. The enzyme showed two polypeptides of approximately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing revealed seven to eight proteins with a pI range of 7.4-8.2. Sedimentation equilibrium yielded a molecular mass of 96 +/- 2 kDa for the enzyme. Carbohydrate analysis revealed that both polypeptides contained Gal, Man, GlcNAc, Fuc, and GalNac saccharides. Like other nucleoside diphosphate kinases, the retinal enzyme showed substantial differences in the Km values for various di- and triphosphate nucleotides. Immunogold labeling of bovine retina revealed that the enzyme is localized on both the membranes and in the cytoplasm. Screening of a retinal cDNA library yielded full-length clones encoding two distinct isoforms (NBR-A and NBR-B). Both isoforms were overexpressed in Escherichia coli and their biochemical properties compared with retinal NDP-kinase. The structures of NBR-A and NBR-B were determined by X-ray crystallography in the presence of guanine nucleotide(s). Both isoforms are hexameric, and the fold of the monomer is similar to other nucleoside diphosphate kinase structures. The NBR-A active site contained both a cGMP and a GDP molecule each bound at half occupancy while the NBR-B active site contained only cGMP.

About this Structure

1BE4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure., Abdulaev NG, Karaschuk GN, Ladner JE, Kakuev DL, Yakhyaev AV, Tordova M, Gaidarov IO, Popov VI, Fujiwara JH, Chinchilla D, Eisenstein E, Gilliland GL, Ridge KD, Biochemistry. 1998 Oct 6;37(40):13958-67. PMID:9760230 Page seeded by OCA on Fri May 2 11:23:46 2008

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