6xte
From Proteopedia
(Difference between revisions)
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==Human karyopherin RanBP5 (isoform-1)== | ==Human karyopherin RanBP5 (isoform-1)== | ||
| - | <StructureSection load='6xte' size='340' side='right'caption='[[6xte]]' scene=''> | + | <StructureSection load='6xte' size='340' side='right'caption='[[6xte]], [[Resolution|resolution]] 2.27Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XTE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XTE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xte]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XTE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XTE FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xte OCA], [http://pdbe.org/6xte PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xte RCSB], [http://www.ebi.ac.uk/pdbsum/6xte PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xte ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=OAR:N-(4-AMINO-5-HYDROXY-PENTYL)-GUANIDINE'>OAR</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FC0:N-CARBOXY-L-PHENYLALANINE'>FC0</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IPO5, KPNB3, RANBP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xte OCA], [http://pdbe.org/6xte PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xte RCSB], [http://www.ebi.ac.uk/pdbsum/6xte PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xte ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/IPO5_HUMAN IPO5_HUMAN]] Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity). In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.[UniProtKB:Q8BKC5]<ref>PMID:9687515</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Here, we describe the crystal structures of two distinct isoforms of ligand-free human karyopherin RanBP5 and investigate its global propensity to interact with influenza A virus polymerase. Our results confirm the general architecture and mechanism of the IMB3 karyopherin-beta subfamily whilst also highlighting differences with the yeast orthologue Kap121p. Moreover, our results provide insight into the structural flexibility of beta-importins in the unbound state. Based on docking of a nuclear localisation sequence, point mutations were designed, which suppress influenza PA-PB1 subcomplex binding to RanBP5 in a binary protein complementation assay. | ||
| + | |||
| + | X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking.,Swale C, Da Costa B, Sedano L, Garzoni F, McCarthy AA, Berger I, Bieniossek C, Ruigrok RWH, Delmas B, Crepin T J Mol Biol. 2020 Mar 25. pii: S0022-2836(20)30254-0. doi:, 10.1016/j.jmb.2020.03.021. PMID:32222384<ref>PMID:32222384</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6xte" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Crepin T]] | + | [[Category: Crepin, T]] |
| - | [[Category: McCarthy | + | [[Category: McCarthy, A A]] |
| - | [[Category: Ruigrok | + | [[Category: Ruigrok, R W.H]] |
| - | [[Category: Swale C]] | + | [[Category: Swale, C]] |
| + | [[Category: Host-pathogen interaction]] | ||
| + | [[Category: Human karyopherin]] | ||
| + | [[Category: Influenza polymerase assembly]] | ||
| + | [[Category: Pa-pb1 sub-complex nuclear import]] | ||
| + | [[Category: Translocase]] | ||
Revision as of 07:42, 27 May 2020
Human karyopherin RanBP5 (isoform-1)
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