5hoo

From Proteopedia

(Difference between revisions)

Revision as of 08:26, 27 May 2020

Crystal structure of the Mos1 Strand Transfer Complex

Structural highlights

5hoo is a 8 chain structure with sequence from Droma. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3hos, 3hot, 4r79, 4u7b
Gene:	mariner (DROMA)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MOS1T_DROMA] Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site.

Publication Abstract from PubMed

Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with transposon ends covalently joined to target DNA, portrays the transposition machinery after DNA integration. It reveals severe distortion of target DNA and flipping of the target adenines into extra-helical positions. Fluorescence experiments confirm dynamic base flipping in solution. Transposase residues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient transposon integration and transposition in vitro. Transposase recognises the flipped target adenines via base-specific interactions with backbone atoms, offering a molecular basis for TA target sequence selection. Our results will provide a template for re-designing mariner/Tc1 transposases with modified target specificities.

A bend, flip and trap mechanism for transposon integration.,Morris ER, Grey H, McKenzie G, Jones AC, Richardson JM Elife. 2016 May 25;5. pii: e15537. doi: 10.7554/eLife.15537. PMID:27223327^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Morris ER, Grey H, McKenzie G, Jones AC, Richardson JM. A bend, flip and trap mechanism for transposon integration. Elife. 2016 May 25;5. pii: e15537. doi: 10.7554/eLife.15537. PMID:27223327 doi:http://dx.doi.org/10.7554/eLife.15537

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5hoo"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Mos1 Strand Transfer Complex==
-<StructureSection load='5hoo' size='340' side='right' caption='[[5hoo]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
+<StructureSection load='5hoo' size='340' side='right'caption='[[5hoo]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5hoo]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5hoo]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Droma Droma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HOO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hos|3hos]], [[3hot|3hot]], [[4r79|4r79]], [[4u7b|4u7b]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hoo OCA], [http://pdbe.org/5hoo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hoo RCSB], [http://www.ebi.ac.uk/pdbsum/5hoo PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mariner ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7226 DROMA])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hoo OCA], [http://pdbe.org/5hoo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hoo RCSB], [http://www.ebi.ac.uk/pdbsum/5hoo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hoo ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 </div>
 <div class="pdbe-citations 5hoo" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Transposase 3D structures|Transposase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Droma]]
+[[Category: Large Structures]]
 [[Category: Morris, E R]]
 [[Category: Richardson, J M]]

5hoo

From Proteopedia

Revision as of 08:26, 27 May 2020

Crystal structure of the Mos1 Strand Transfer Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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