1bf4

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[[Image:1bf4.gif|left|200px]]
[[Image:1bf4.gif|left|200px]]
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{{Structure
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|PDB= 1bf4 |SIZE=350|CAPTION= <scene name='initialview01'>1bf4</scene>, resolution 1.6&Aring;
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The line below this paragraph, containing "STRUCTURE_1bf4", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=5IU:5-IODO-2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_1bf4| PDB=1bf4 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bf4 OCA], [http://www.ebi.ac.uk/pdbsum/1bf4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bf4 RCSB]</span>
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'''CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX'''
'''CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX'''
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[[Category: Su, S.]]
[[Category: Su, S.]]
[[Category: Wang, A H.J.]]
[[Category: Wang, A H.J.]]
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[[Category: archaebacteria]]
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[[Category: Archaebacteria]]
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[[Category: complex (dna-binding protein/dna)]]
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[[Category: Dna binding protein]]
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[[Category: dna binding protein]]
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[[Category: Hyperthermophile]]
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[[Category: hyperthermophile]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:25:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:59:47 2008''
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Revision as of 08:26, 2 May 2008

Image:1bf4.gif

Template:STRUCTURE 1bf4

CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX


Overview

Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing.

About this Structure

1BF4 is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA.

Reference

The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA., Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary BS, Edmondson SP, Shriver JW, Wang AH, Nat Struct Biol. 1998 Sep;5(9):782-6. PMID:9731772 Page seeded by OCA on Fri May 2 11:25:59 2008

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