1bfu
From Proteopedia
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'''SUBTILISIN CARLSBERG IN 20% DIOXANE''' | '''SUBTILISIN CARLSBERG IN 20% DIOXANE''' | ||
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[[Category: Schmitke, J L.]] | [[Category: Schmitke, J L.]] | ||
[[Category: Stern, L J.]] | [[Category: Stern, L J.]] | ||
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| - | [[Category: | + | [[Category: Organic solvent]] |
| - | [[Category: | + | [[Category: Serine protease]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:27:31 2008'' | |
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Revision as of 08:27, 2 May 2008
SUBTILISIN CARLSBERG IN 20% DIOXANE
Overview
The X-ray crystal structures of the protease subtilisin Carlsberg in 40% acetonitrile and in 20% dioxane have been determined to at least 2.3 A resolution, and their solvent binding patterns have been compared to those observed in the neat organic solvents. The structures of the protein in the two aqueous-organic mixtures are essentially the same as in pure water, acetonitrile, and dioxane. Interestingly, the enzyme-bound organic solvent molecules tend to congregate in the active site. Three of the five bound acetonitrile molecules observed in the structure of subtilisin in 40% acetonitrile are situated in the enzyme active site, as is the single enzyme-bound dioxane molecule observed in 20% dioxane (whose location is distinct from that of any bound acetonitrile molecule). Furthermore, the organic solvent molecules detected in the enzyme active site in the aqueous-organic mixtures are in the same locations as in the structures in the corresponding neat organic solvents.
About this Structure
1BFU is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.
Reference
Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents., Schmitke JL, Stern LJ, Klibanov AM, Biochem Biophys Res Commun. 1998 Jul 20;248(2):273-7. PMID:9675126 Page seeded by OCA on Fri May 2 11:27:31 2008
