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6xwy

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Revision as of 06:43, 3 June 2020

Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed

Structural highlights

6xwy is a 4 chain structure with sequence from Bubble-tip anemone. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RFP_ENTQU] Pigment protein.^[1]

Publication Abstract from PubMed

Engineered proteins must be phenotypically selected for function in the appropriate physiological context. Here, we present a versatile approach that allows generating panels of mammalian cells that express diversified heterologous protein libraries in the cytosol or subcellular compartments under stable conditions and in a single-variant-per-cell manner. To this end we adapt CRISPR/Cas9 editing technology to diversify targeted stretches of a protein of interest in situ. We demonstrate the utility of the approach by in situ engineering and intra-lysosome specific selection of an extremely pH-resistant long Stokes shift red fluorescent protein variant. Tailoring properties to specific conditions of cellular sub-compartments or organelles of mammalian cells can be an important asset to optimize various proteins, protein-based tools, and biosensors for distinct functions.

Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells.,Erdogan M, Fabritius A, Basquin J, Griesbeck O Cell Chem Biol. 2020 Mar 3. pii: S2451-9456(20)30068-4. doi:, 10.1016/j.chembiol.2020.02.004. PMID:32142629^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wiedenmann J, Schenk A, Rocker C, Girod A, Spindler KD, Nienhaus GU. A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria). Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11646-51. Epub 2002 Aug 15. PMID:12185250 doi:http://dx.doi.org/10.1073/pnas.182157199
↑ Erdogan M, Fabritius A, Basquin J, Griesbeck O. Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells. Cell Chem Biol. 2020 Mar 3. pii: S2451-9456(20)30068-4. doi:, 10.1016/j.chembiol.2020.02.004. PMID:32142629 doi:http://dx.doi.org/10.1016/j.chembiol.2020.02.004

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6xwy"

@@ Line 3: / Line 3: @@
 <StructureSection load='6xwy' size='340' side='right'caption='[[6xwy]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6xwy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6XWY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6xwy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XWY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6xwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwy OCA], [http://pdbe.org/6xwy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xwy RCSB], [http://www.ebi.ac.uk/pdbsum/6xwy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwy OCA], [http://pdbe.org/6xwy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xwy RCSB], [http://www.ebi.ac.uk/pdbsum/6xwy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwy ProSAT]</span></td></tr>
 </table>
 == Function ==

6xwy

From Proteopedia

Revision as of 06:43, 3 June 2020

Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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