1bhc

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[[Image:1bhc.jpg|left|200px]]
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{{Structure
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|LIGAND= <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhc OCA], [http://www.ebi.ac.uk/pdbsum/1bhc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bhc RCSB]</span>
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'''BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE'''
'''BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE'''
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[[Category: Hamiaux, C.]]
[[Category: Hamiaux, C.]]
[[Category: Prange, T.]]
[[Category: Prange, T.]]
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[[Category: protease inhibitor]]
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[[Category: Protease inhibitor]]
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[[Category: trypsin]]
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[[Category: Trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:30:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:01:01 2008''
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Revision as of 08:30, 2 May 2008

Image:1bhc.jpg

Template:STRUCTURE 1bhc

BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE


Overview

The structure of a monoclinic form of bovine pancreatic trypsin inhibitor (BPTI) crystallized from a thiocyanate solution has been determined and refined at 2.7 A resolution. The space group is P21 with a = 71.56, b = 73.83, c = 64.47 A, beta = 93.9 degrees and Z = 20. The ten independent molecules were located by a multi-body molecular-replacement search as developed in the AMoRe program, starting from a single monomer model (PDB code: 6PTI). The molecular arrangement of the subunits is a decamer resulting from the combination of two orthogonal fivefold and twofold non-crystallographic axes. This builds a globular micelle-like particle which minimizes hydrophobic interactions with the solvent. The refinement was conducted with non-crystallographic symmetry constraints up to a final residual of R = 0.20 (Rfree= 0.26). The root-mean-square deviations from ideal geometry were 0.015 A and 1.6 degrees on bond distances and bond angles, respectively. Several sites for thiocyanate ions were analyzed.

About this Structure

1BHC is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The decameric structure of bovine pancreatic trypsin inhibitor (BPTI) crystallized from thiocyanate at 2.7 A resolution., Hamiaux C, Prange T, Ries-Kautt M, Ducruix A, Lafont S, Astier JP, Veesler S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):103-13. Epub 1999, Jan 1. PMID:10089400 Page seeded by OCA on Fri May 2 11:30:52 2008

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