6sgu
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc== | ==Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc== | ||
| - | <StructureSection load='6sgu' size='340' side='right'caption='[[6sgu]]' scene=''> | + | <StructureSection load='6sgu' size='340' side='right'caption='[[6sgu]], [[Resolution|resolution]] 3.27Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SGU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SGU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sgu]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SGU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SGU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sgu OCA], [http://pdbe.org/6sgu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sgu RCSB], [http://www.ebi.ac.uk/pdbsum/6sgu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sgu ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">acrB, acrE, b0462, JW0451 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sgu OCA], [http://pdbe.org/6sgu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sgu RCSB], [http://www.ebi.ac.uk/pdbsum/6sgu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sgu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI]] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The small protein AcrZ in Escherichia coli interacts with the transmembrane portion of the multidrug efflux pump AcrB and increases resistance of the bacterium to a subset of the antibiotic substrates of that transporter. It is not clear how the physical association of the two proteins selectively changes activity of the pump for defined substrates. Here, we report cryo-EM structures of AcrB and the AcrBZ complex in lipid environments, and comparisons suggest that conformational changes occur in the drug-binding pocket as a result of AcrZ binding. Simulations indicate that cardiolipin preferentially interacts with the AcrBZ complex, due to increased contact surface, and we observe that chloramphenicol sensitivity of bacteria lacking AcrZ is exacerbated when combined with cardiolipin deficiency. Taken together, the data suggest that AcrZ and lipid cooperate to allosterically modulate AcrB activity. This mode of regulation by a small protein and lipid may occur for other membrane proteins. | ||
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| + | Interactions of a Bacterial RND Transporter with a Transmembrane Small Protein in a Lipid Environment.,Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF Structure. 2020 Apr 16. pii: S0969-2126(20)30095-2. doi:, 10.1016/j.str.2020.03.013. PMID:32348749<ref>PMID:32348749</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6sgu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Ecoli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Du D]] | + | [[Category: Synthetic construct sequences]] |
| - | [[Category: Luisi | + | [[Category: Du, D]] |
| - | [[Category: Neuberger A]] | + | [[Category: Luisi, B F]] |
| - | [[Category: Newman C]] | + | [[Category: Neuberger, A]] |
| - | [[Category: Szewczak-Harris A]] | + | [[Category: Newman, C]] |
| + | [[Category: Szewczak-Harris, A]] | ||
| + | [[Category: Antibiotic resistance]] | ||
| + | [[Category: Drug transport]] | ||
| + | [[Category: Efflux pump]] | ||
| + | [[Category: Lipid nanodisc]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Rnd transporter]] | ||
Revision as of 06:40, 10 June 2020
Cryo-EM structure of Escherichia coli AcrB and DARPin in Saposin A-nanodisc
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