5j7p

From Proteopedia

(Difference between revisions)

Revision as of 07:32, 10 June 2020

Macrophage Migration Inhibitory Factor bound to Covalent Inhibitor RDR03785

Structural highlights

5j7p is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	5j7q
Gene:	MIF, GLIF, MMIF (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[MIF_HUMAN] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

Function

[MIF_HUMAN] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.^[1] ^[2]

Publication Abstract from PubMed

Inhibitors of human macrophage migration inhibitory factor (MIF) previously reported in the literature have been reexamined by synthesis, assaying for tautomerase activity, and protein crystallography. Substantial inconsistencies between prior and current assay results are noted. They appear to arise from difficulties with the tautomerase substrates, solubility issues, and especially covalent inhibition. Incubation time variation shows that 3, 4, 6, and 9 are covalent or slow-binding inhibitors. Two protein crystal structures are provided; one confirms that the twice-discovered 3 is a covalent inhibitor.

Irregularities in enzyme assays: The case of macrophage migration inhibitory factor.,Cisneros JA, Robertson MJ, Valhondo M, Jorgensen WL Bioorg Med Chem Lett. 2016 Jun 15;26(12):2764-7. doi: 10.1016/j.bmcl.2016.04.074., Epub 2016 Apr 26. PMID:27156768^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oddo M, Calandra T, Bucala R, Meylan PR. Macrophage migration inhibitory factor reduces the growth of virulent Mycobacterium tuberculosis in human macrophages. Infect Immun. 2005 Jun;73(6):3783-6. PMID:15908412 doi:10.1128/IAI.73.6.3783-3786.2005
↑ Emonts M, Sweep FC, Grebenchtchikov N, Geurts-Moespot A, Knaup M, Chanson AL, Erard V, Renner P, Hermans PW, Hazelzet JA, Calandra T. Association between high levels of blood macrophage migration inhibitory factor, inappropriate adrenal response, and early death in patients with severe sepsis. Clin Infect Dis. 2007 May 15;44(10):1321-8. Epub 2007 Apr 5. PMID:17443469 doi:10.1086/514344
↑ Cisneros JA, Robertson MJ, Valhondo M, Jorgensen WL. Irregularities in enzyme assays: The case of macrophage migration inhibitory factor. Bioorg Med Chem Lett. 2016 Jun 15;26(12):2764-7. doi: 10.1016/j.bmcl.2016.04.074., Epub 2016 Apr 26. PMID:27156768 doi:http://dx.doi.org/10.1016/j.bmcl.2016.04.074

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5j7p"

@@ Line 1: / Line 1: @@
 ==Macrophage Migration Inhibitory Factor bound to Covalent Inhibitor RDR03785==
-<StructureSection load='5j7p' size='340' side='right' caption='[[5j7p]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+<StructureSection load='5j7p' size='340' side='right'caption='[[5j7p]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5j7p]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J7P FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5j7p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J7P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J7P FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6H1:6-{[4-(TRIFLUOROMETHYL)PHENYL]METHYL}-2H-1,3-BENZODIOXOL-5-OL'>6H1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6H1:6-{[4-(TRIFLUOROMETHYL)PHENYL]METHYL}-2H-1,3-BENZODIOXOL-5-OL'>6H1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5j7q|5j7q]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j7p OCA], [http://pdbe.org/5j7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j7p RCSB], [http://www.ebi.ac.uk/pdbsum/5j7p PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MIF, GLIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j7p OCA], [http://pdbe.org/5j7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j7p RCSB], [http://www.ebi.ac.uk/pdbsum/5j7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j7p ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 21: / Line 22: @@
 </div>
 <div class="pdbe-citations 5j7p" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Macrophage inhibitory factor 3D structures|Macrophage inhibitory factor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Jorgensen, W L]]
 [[Category: Robertson, M J]]

5j7p

From Proteopedia

Revision as of 07:32, 10 June 2020

Macrophage Migration Inhibitory Factor bound to Covalent Inhibitor RDR03785

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox