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5jdb

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Revision as of 07:43, 10 June 2020

Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens

Structural highlights

5jdb is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[E2EA82_9REOV] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.[SAAS:SAAS00136874] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.[SAAS:SAAS00136880]

Publication Abstract from PubMed

RotaTeq((R)) and Rotarix are two common human rotavirus (RV) vaccines currently on the market worldwide. Recent studies indicate histo-blood group antigens (HBGAs) may be attachment factors for RVs. The P[8] VP8* proteins of RotaTeq and Rotarix were expressed and purified, and their binding specificities were evaluated. Saliva-based binding assays showed that the VP8* proteins bound to the saliva samples of secretors irrespective of ABO blood types. However, in the oligosaccharide binding assay, the VP8* proteins displayed no specific binding to the HBGAs tested, including Lewis b and H1. The structure of RotaTeq P[8] VP8* was solved at 1.9A. Structural comparisons revealed that the putative receptor binding site was different to that of other genotypes and displayed a novel potential binding region. These findings indicate RotaTeq and Rotarix may have better efficiency in areas with a high percentage of secretors.

Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens.,Sun X, Guo N, Li D, Jin M, Zhou Y, Xie G, Pang L, Zhang Q, Cao Y, Duan ZJ Virology. 2016 Aug;495:129-35. doi: 10.1016/j.virol.2016.05.010. Epub 2016 May, 19. PMID:27209447^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sun X, Guo N, Li D, Jin M, Zhou Y, Xie G, Pang L, Zhang Q, Cao Y, Duan ZJ. Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens. Virology. 2016 Aug;495:129-35. doi: 10.1016/j.virol.2016.05.010. Epub 2016 May, 19. PMID:27209447 doi:http://dx.doi.org/10.1016/j.virol.2016.05.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5jdb"

@@ Line 1: / Line 1: @@
 ==Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens==
-<StructureSection load='5jdb' size='340' side='right' caption='[[5jdb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='5jdb' size='340' side='right'caption='[[5jdb]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5jdb]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JDB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5jdb]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JDB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JDB FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jdb OCA], [http://pdbe.org/5jdb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jdb RCSB], [http://www.ebi.ac.uk/pdbsum/5jdb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jdb ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jdb OCA], [http://pdbe.org/5jdb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jdb RCSB], [http://www.ebi.ac.uk/pdbsum/5jdb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jdb ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Cao, Y]]
 [[Category: Duan, Z]]

5jdb

From Proteopedia

Revision as of 07:43, 10 June 2020

Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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