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2uwc

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Revision as of 11:12, 17 June 2020

Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2

Structural highlights

2uwc is a 2 chain structure with sequence from Common nasturtium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2uwb
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q07524_TROMA] Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.[RuleBase:RU361120]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen (Populus tremula x Populus tremuloides). Production of the loop deletion variant Tm-NXG1-DeltaYNIIG yielded an enzyme that was structurally similar to Ptt-XET16-34 and had a greatly increased transglycosylation:hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.

Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism.,Baumann MJ, Eklof JM, Michel G, Kallas AM, Teeri TT, Czjzek M, Brumer H 3rd Plant Cell. 2007 Jun;19(6):1947-63. Epub 2007 Jun 8. PMID:17557806^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Baumann MJ, Eklof JM, Michel G, Kallas AM, Teeri TT, Czjzek M, Brumer H 3rd. Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism. Plant Cell. 2007 Jun;19(6):1947-63. Epub 2007 Jun 8. PMID:17557806 doi:10.1105/tpc.107.051391

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uwc"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2==
-<StructureSection load='2uwc' size='340' side='right' caption='[[2uwc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='2uwc' size='340' side='right'caption='[[2uwc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2uwc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_nasturtium Common nasturtium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UWC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2uwc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_nasturtium Common nasturtium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2UWC FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2uwb|2uwb]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwc OCA], [http://pdbe.org/2uwc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uwc RCSB], [http://www.ebi.ac.uk/pdbsum/2uwc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwc ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2uwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwc OCA], [http://pdbe.org/2uwc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uwc RCSB], [http://www.ebi.ac.uk/pdbsum/2uwc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwc ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Common nasturtium]]
+[[Category: Large Structures]]
 [[Category: Baumann, M J]]
 [[Category: Brumer, H]]

2uwc

From Proteopedia

Revision as of 11:12, 17 June 2020

Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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