This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1bln
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1bln.gif|left|200px]] | [[Image:1bln.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1bln", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | | | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | + | --> | |
| - | + | {{STRUCTURE_1bln| PDB=1bln | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''ANTI-P-GLYCOPROTEIN FAB MRK-16''' | '''ANTI-P-GLYCOPROTEIN FAB MRK-16''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BLN OCA]. | |
==Reference== | ==Reference== | ||
Mode of binding of anti-P-glycoprotein antibody MRK-16 to its antigen. A crystallographic and molecular modeling study., Vasudevan S, Tsuruo T, Rose DR, J Biol Chem. 1998 Sep 25;273(39):25413-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9738009 9738009] | Mode of binding of anti-P-glycoprotein antibody MRK-16 to its antigen. A crystallographic and molecular modeling study., Vasudevan S, Tsuruo T, Rose DR, J Biol Chem. 1998 Sep 25;273(39):25413-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9738009 9738009] | ||
| - | [[Category: Mus musculus]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Rose, D R.]] | [[Category: Rose, D R.]] | ||
[[Category: Tsuruo, T.]] | [[Category: Tsuruo, T.]] | ||
[[Category: Vasudevan, S.]] | [[Category: Vasudevan, S.]] | ||
| - | [[Category: | + | [[Category: Immunoglobulin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:40:27 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:40, 2 May 2008
ANTI-P-GLYCOPROTEIN FAB MRK-16
Overview
Monoclonal antibody MRK-16 recognizes a discontinuous extracellular epitope on the multidrug resistance-associated ATP-binding cassette transporter, P-glycoprotein. The atomic basis for specificity of this antibody is of interest because of its potential as a modulator of P-glycoprotein activity. The crystal structure of Fab MRK-16 is reported to a resolution of 2.8 A. A structure for a portion of the epitope was derived by comparison to regions of solved structures with similar primary sequence. This has permitted a proposal for the mode of binding of the peptide epitope to the antibody, in which the peptide makes specific contacts with complementarity-determining regions H1, H2, and H3 from the heavy chain and L3 from the light chain. These interactions are consistent with epitope mapping studies and with the observation that MRK-16 is specific for human class I P-glycoprotein. This result identifies side chains in MRK-16 that would be amenable to alteration in antibody engineering experiments to derive improved multidrug resistance inhibitors for clinical use during chemotherapy. In particular, Arg-H97 contacts both Glu-746 and Asp-744 of the peptide, Arg-L96 contacts Asp-743, and Thr-H33 interacts with Thr-747. All of these epitope residues were implicated in mediating specificity by epitope mapping studies.
About this Structure
Full crystallographic information is available from OCA.
Reference
Mode of binding of anti-P-glycoprotein antibody MRK-16 to its antigen. A crystallographic and molecular modeling study., Vasudevan S, Tsuruo T, Rose DR, J Biol Chem. 1998 Sep 25;273(39):25413-9. PMID:9738009 Page seeded by OCA on Fri May 2 11:40:27 2008
