1br3
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1br3.gif|left|200px]] | [[Image:1br3.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1br3", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1br3| PDB=1br3 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME''' | '''CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR3 OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme., Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF, Nat Struct Biol. 1999 Feb;6(2):151-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10048927 10048927] | Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme., Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF, Nat Struct Biol. 1999 Feb;6(2):151-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10048927 10048927] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Joyce, G F.]] | [[Category: Joyce, G F.]] | ||
[[Category: Nowakowski, J.]] | [[Category: Nowakowski, J.]] | ||
| Line 29: | Line 25: | ||
[[Category: Shim, P J.]] | [[Category: Shim, P J.]] | ||
[[Category: Stout, C D.]] | [[Category: Stout, C D.]] | ||
| - | [[Category: | + | [[Category: Dna enzyme]] |
| - | [[Category: | + | [[Category: Holliday junction]] |
| - | [[Category: | + | [[Category: Ribozyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:51:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:51, 2 May 2008
CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME
Overview
The structure of a large nucleic acid complex formed by the 10-23 DNA enzyme bound to an RNA substrate was determined by X-ray diffraction at 3.0 A resolution. The 82-nucleotide complex contains two strands of DNA and two strands of RNA that form five double-helical domains. The spatial arrangement of these helices is maintained by two four-way junctions that exhibit extensive base-stacking interactions and sharp turns of the phosphodiester backbone stabilized by metal ions coordinated to nucleotides at these junctions. Although it is unlikely that the structure corresponds to the catalytically active conformation of the enzyme, it represents a novel nucleic acid fold with implications for the Holliday junction structure.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme., Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF, Nat Struct Biol. 1999 Feb;6(2):151-6. PMID:10048927 Page seeded by OCA on Fri May 2 11:51:22 2008
