1bvr

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[[Image:1bvr.gif|left|200px]]
[[Image:1bvr.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1bvr |SIZE=350|CAPTION= <scene name='initialview01'>1bvr</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_1bvr", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=THT:TRANS-2-HEXADECENOYL-(N-ACETYL-CYSTEAMINE)-THIOESTER'>THT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0623 FabI], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK07889 PRK07889]</span>
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{{STRUCTURE_1bvr| PDB=1bvr | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bvr OCA], [http://www.ebi.ac.uk/pdbsum/1bvr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bvr RCSB]</span>
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'''M.TB. ENOYL-ACP REDUCTASE (INHA) IN COMPLEX WITH NAD+ AND C16-FATTY-ACYL-SUBSTRATE'''
'''M.TB. ENOYL-ACP REDUCTASE (INHA) IN COMPLEX WITH NAD+ AND C16-FATTY-ACYL-SUBSTRATE'''
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==Reference==
==Reference==
Crystal structure of the Mycobacterium tuberculosis enoyl-ACP reductase, InhA, in complex with NAD+ and a C16 fatty acyl substrate., Rozwarski DA, Vilcheze C, Sugantino M, Bittman R, Sacchettini JC, J Biol Chem. 1999 May 28;274(22):15582-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10336454 10336454]
Crystal structure of the Mycobacterium tuberculosis enoyl-ACP reductase, InhA, in complex with NAD+ and a C16 fatty acyl substrate., Rozwarski DA, Vilcheze C, Sugantino M, Bittman R, Sacchettini JC, J Biol Chem. 1999 May 28;274(22):15582-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10336454 10336454]
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[[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
 
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: Vilcheze, C.]]
[[Category: Vilcheze, C.]]
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[[Category: nadh-dependent enoyl-acp reductase]]
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[[Category: Nadh-dependent enoyl-acp reductase]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: tb structural genomics consortium]]
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[[Category: Tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: Tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:00:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:09:17 2008''
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Revision as of 09:00, 2 May 2008

Image:1bvr.gif

Template:STRUCTURE 1bvr

M.TB. ENOYL-ACP REDUCTASE (INHA) IN COMPLEX WITH NAD+ AND C16-FATTY-ACYL-SUBSTRATE


Overview

Enoyl-ACP reductases participate in fatty acid biosynthesis by utilizing NADH to reduce the trans double bond between positions C2 and C3 of a fatty acyl chain linked to the acyl carrier protein. The enoyl-ACP reductase from Mycobacterium tuberculosis, known as InhA, is a member of an unusual FAS-II system that prefers longer chain fatty acyl substrates for the purpose of synthesizing mycolic acids, a major component of mycobacterial cell walls. The crystal structure of InhA in complex with NAD+ and a C16 fatty acyl substrate, trans-2-hexadecenoyl-(N-acetylcysteamine)-thioester, reveals that the substrate binds in a general "U-shaped" conformation, with the trans double bond positioned directly adjacent to the nicotinamide ring of NAD+. The side chain of Tyr158 directly interacts with the thioester carbonyl oxygen of the C16 fatty acyl substrate and therefore could help stabilize the enolate intermediate, proposed to form during substrate catalysis. Hydrophobic residues, primarily from the substrate binding loop (residues 196-219), engulf the fatty acyl chain portion of the substrate. The substrate binding loop of InhA is longer than that of other enoyl-ACP reductases and creates a deeper substrate binding crevice, consistent with the ability of InhA to recognize longer chain fatty acyl substrates.

About this Structure

1BVR is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Mycobacterium tuberculosis enoyl-ACP reductase, InhA, in complex with NAD+ and a C16 fatty acyl substrate., Rozwarski DA, Vilcheze C, Sugantino M, Bittman R, Sacchettini JC, J Biol Chem. 1999 May 28;274(22):15582-9. PMID:10336454 Page seeded by OCA on Fri May 2 12:00:48 2008

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