1gne
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(New page: 200px<br /> <applet load="1gne" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gne, resolution 2.5Å" /> '''THE THREE-DIMENSIONA...)
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Revision as of 11:58, 8 November 2007
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THE THREE-DIMENSIONAL STRUCTURE OF GLUTATHIONE S-TRANSFERASE OF SCHISTOSOMA JAPONICUM FUSED WITH A CONSERVED NEUTRALIZING EPITOPE ON GP41 OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
Overview
The 3-dimensional crystal structure of glutathione S-transferase (GST) of, Schistosoma japonicum (Sj) fused with a conserved neutralizing epitope on, gp41 (glycoprotein, 41 kDa) of human immunodeficiency virus type 1 (HIV-1), (Muster T et al., 1993, J Virol 67:6642-6647) was determined at 2.5 A, resolution. The structure of the 3-3 isozyme rat GST of the mu gene class, (Ji X, Zhang P, Armstrong RN, Gilliland GL, 1992, Biochemistry, 31:10169-10184) was used as a molecular replacement model. The structure, consists of a 4-stranded beta-sheet and 3 alpha-helices in domain 1 and 5, alpha-helices in domain 2. The space group of the Sj GST crystal is, P4(3)2(1)2, with unit cell dimensions of a = b = 94.7 A, and c = 58.1 A., The crystal has 1 GST monomer per asymmetric unit, and 2 monomers that, form an active dimer are related by crystallographic 2-fold symmetry. In, the binding site, the ordered structure of reduced glutathione is, observed. The gp41 peptide (Glu-Leu-Asp-Lys-Trp-Ala) fused to the, C-terminus of Sj GST forms a loop stabilized by symmetry-related GSTs. The, Sj GST structure is compared with previously determined GST structures of, mammalian gene classes mu, alpha, and pi. Conserved amino acid residues, among the 4 GSTs that are important for hydrophobic and hydrophilic, interactions for dimer association and glutathione binding are discussed.
About this Structure
1GNE is a Single protein structure of sequence from Synthetic construct with GSW as ligand. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV., Lim K, Ho JX, Keeling K, Gilliland GL, Ji X, Ruker F, Carter DC, Protein Sci. 1994 Dec;3(12):2233-44. PMID:7538846
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