1by0
From Proteopedia
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[[Image:1by0.gif|left|200px]] | [[Image:1by0.gif|left|200px]] | ||
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'''N-TERMINAL LEUCINE-REPEAT REGION OF HEPATITIS DELTA ANTIGEN''' | '''N-TERMINAL LEUCINE-REPEAT REGION OF HEPATITIS DELTA ANTIGEN''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BY0 is a [[Single protein]] structure | + | 1BY0 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BY0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lin, I J.]] | [[Category: Lin, I J.]] | ||
[[Category: Lou, Y C.]] | [[Category: Lou, Y C.]] | ||
| - | [[Category: | + | [[Category: Helix]] |
| - | [[Category: | + | [[Category: Hepatitis delta antigen]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Rna binding]] |
| - | [[Category: | + | [[Category: Solution structure]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:06:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:06, 2 May 2008
N-TERMINAL LEUCINE-REPEAT REGION OF HEPATITIS DELTA ANTIGEN
Overview
Hepatitis delta virus (HDV) is a satellite virus of the hepatitis B virus (HBV) which provides the surface antigen for the viral coat. The RNA genome of HDV encodes two proteins: the small delta antigen and the large delta antigen. The two proteins resemble each other except for the presence of an additional 19 amino acids at the C terminus of the latter species. We have found that the N-terminal leucine-repeat region of hepatitis delta antigen (HDAg) binds to the autolytic domain of HDV genomic RNA and attenuates its autolytic activity. A 27-residue polypeptide corresponding to residues 24-50 of HDAg, designated dAg(24-50), was synthesized, and its solution structure was found to be an alpha-helix by circular dichroism and (1)H-nuclear magnetic resonance (NMR) techniques. Binding affinity of dAg(24-50) with HDV genomic RNA was found to increase with its alpha-helical content, and it was further confirmed by modifying its N- and C-terminal groups. Furthermore, the absence of RNA binding activity in the mutant peptides, dAgM(24-50am) and dAgM(Ac24-50am), in which Lys38, Lys39, and Lys40 were changed to Glu, indicates a possible involvement of these residues in their binding activity. Structural knowledge of the N-terminal leucine-repeat region of HDAg thus provides a molecular basis for the understanding of its role in the interaction with RNA. Proteins 1999;37:121-129.
About this Structure
1BY0 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Solution structure and RNA-binding activity of the N-terminal leucine-repeat region of hepatitis delta antigen., Lin IJ, Lou YC, Pai MT, Wu HN, Cheng JW, Proteins. 1999 Oct 1;37(1):121-9. PMID:10451556 Page seeded by OCA on Fri May 2 12:06:01 2008
