2w48

From Proteopedia

(Difference between revisions)

Revision as of 08:55, 25 June 2020

Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae

Structural highlights

2w48 is a 4 chain structure with sequence from "bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SORC_KLEPN] Positively regulates, in the presence of L-sorbose, and negatively regulates, in the absence of L-sorbose, the transcription of the sor operon.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

SorC transcriptional regulators are common regulators in prokaryotes. Here we report the first crystal structure of a full-length SorC, the sorbitol operon regulator SorC from Klebsiella pneumoniae, the prototype of its family. SorC was found to be a homotetramer (which seems to be the biologically active form) that is able to recognize its DNA operator. The tetramer can be regarded as a dimer of dimers, with each dimer being composed of two identical subunits in different conformations. The DNA-binding domains divergently protrude from the core of the tetramer, suggesting that SorC may bind its operator in two distinct regions. The sugar-binding domain presents the same fold identified in members of the SorC family that shows some features identified as specific for sugar recognition. An in silico analysis shows that the localization of the putative sugar-binding site is close to the dimeric interfaces. This supports the proposal of a new mechanism of transcriptional regulation that is in complete agreement with functional studies recently reported on a protein belonging to the same family.

Crystal structure of the full-length sorbitol operon regulator SorC from Klebsiella pneumoniae: structural evidence for a novel transcriptional regulation mechanism.,de Sanctis D, McVey CE, Enguita FJ, Carrondo MA J Mol Biol. 2009 Apr 3;387(3):759-70. Epub 2009 Feb 14. PMID:19232357^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ de Sanctis D, McVey CE, Enguita FJ, Carrondo MA. Crystal structure of the full-length sorbitol operon regulator SorC from Klebsiella pneumoniae: structural evidence for a novel transcriptional regulation mechanism. J Mol Biol. 2009 Apr 3;387(3):759-70. Epub 2009 Feb 14. PMID:19232357 doi:10.1016/j.jmb.2009.02.017

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w48"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae==
-<StructureSection load='2w48' size='340' side='right' caption='[[2w48]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+<StructureSection load='2w48' size='340' side='right'caption='[[2w48]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w48]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W48 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w48]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W48 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W48 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w48 OCA], [http://pdbe.org/2w48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w48 RCSB], [http://www.ebi.ac.uk/pdbsum/2w48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w48 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w48 OCA], [http://pdbe.org/2w48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w48 RCSB], [http://www.ebi.ac.uk/pdbsum/2w48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w48 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Carrondo, M A]]
 [[Category: Enguita, F J]]

2w48

From Proteopedia

Revision as of 08:55, 25 June 2020

Crystal structure of the Full-length Sorbitol Operon Regulator SorC from Klebsiella pneumoniae

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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