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2v64

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Revision as of 08:10, 1 July 2020

Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.

Structural highlights

2v64 is a 9 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1duj, 1go4, 1klq, 1s2h
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The 25 kDa Mad2 protein is a key player in the spindle assembly checkpoint, a safeguard against chromosome segregation errors in mitosis. Mad2 combines three unusual properties. First, Mad2 adopts two conformations with distinct topologies, open (O) and closed (C) Mad2. Second, C-Mad2 forms topological links with its two best-characterized protein ligands, Mad1 and Cdc20. Third, O-Mad2 and C-Mad2 engage in a "conformational" dimer that is essential for spindle checkpoint function in different organisms. The crystal structure of the O-Mad2-C-Mad2 conformational dimer, reported here, reveals an asymmetric interface that explains the selective dimerization of the O-Mad2 and C-Mad2 conformers. The structure also identifies several buried hydrophobic residues whose rearrangement correlates with the Mad2 topological change. The structure of the O-Mad2-C-Mad2 conformational dimer is consistent with a catalytic model in which a C-Mad2 template facilitates the binding of O-Mad2 to Cdc20, the target of Mad2 in the spindle checkpoint.

The Mad2 conformational dimer: structure and implications for the spindle assembly checkpoint.,Mapelli M, Massimiliano L, Santaguida S, Musacchio A Cell. 2007 Nov 16;131(4):730-43. PMID:18022367^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mapelli M, Massimiliano L, Santaguida S, Musacchio A. The Mad2 conformational dimer: structure and implications for the spindle assembly checkpoint. Cell. 2007 Nov 16;131(4):730-43. PMID:18022367 doi:10.1016/j.cell.2007.08.049

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v64"

@@ Line 1: / Line 1: @@
 ==Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.==
-<StructureSection load='2v64' size='340' side='right' caption='[[2v64]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='2v64' size='340' side='right'caption='[[2v64]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2v64]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V64 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V64 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2v64]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V64 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2V64 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1duj|1duj]], [[1go4|1go4]], [[1klq|1klq]], [[1s2h|1s2h]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v64 OCA], [http://pdbe.org/2v64 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v64 RCSB], [http://www.ebi.ac.uk/pdbsum/2v64 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v64 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2v64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v64 OCA], [http://pdbe.org/2v64 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v64 RCSB], [http://www.ebi.ac.uk/pdbsum/2v64 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v64 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Mapelli, M]]
 [[Category: Massimiliano, L]]

2v64

From Proteopedia

Revision as of 08:10, 1 July 2020

Crystallographic structure of the conformational dimer of the Spindle Assembly Checkpoint protein Mad2.

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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