This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1byl
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1byl.jpg|left|200px]] | [[Image:1byl.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1byl", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1byl| PDB=1byl | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS''' | '''BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS''' | ||
| Line 29: | Line 26: | ||
[[Category: Dumas, P.]] | [[Category: Dumas, P.]] | ||
[[Category: Masson, J M.]] | [[Category: Masson, J M.]] | ||
| - | [[Category: | + | [[Category: Antibiotic resistance]] |
| - | [[Category: | + | [[Category: Bleomycin]] |
| - | [[Category: | + | [[Category: Chain swapping]] |
| - | [[Category: | + | [[Category: Drug sequestering]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:07:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:07, 2 May 2008
BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS
Overview
The antibiotic bleomycin, a strong DNA cutting agent, is naturally produced by actinomycetes which have developed a resistance mechanism against such a lethal compound. The crystal structure, at 2.3 A resolution, of a bleomycin resistance protein of 14 kDa reveals a structure in two halves with the same alpha/beta fold despite no sequence similarity. The crystal packing shows compact dimers with a hydrophobic interface and involved in mutual chain exchange. Two independent solution studies (analytical centrifugation and light scattering) showed that this dimeric form is not a packing artefact but is indeed the functional one. Furthermore, light scattering also showed that one dimer binds two antibiotic molecules as expected. A crevice located at the dimer interface, as well as the results of a site-directed mutagenesis study, led to a model wherein two bleomycin molecules are completely sequestered by one dimer. This provides a novel insight into antibiotic resistance due to drug sequestering, and probably also into drug transport and excretion.
About this Structure
1BYL is a Single protein structure of sequence from Streptoalloteichus hindustanus. Full crystallographic information is available from OCA.
Reference
Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering., Dumas P, Bergdoll M, Cagnon C, Masson JM, EMBO J. 1994 Jun 1;13(11):2483-92. PMID:7516875 Page seeded by OCA on Fri May 2 12:07:17 2008
