1byp

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[[Image:1byp.jpg|left|200px]]
[[Image:1byp.jpg|left|200px]]
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{{Structure
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|PDB= 1byp |SIZE=350|CAPTION= <scene name='initialview01'>1byp</scene>, resolution 1.75&Aring;
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The line below this paragraph, containing "STRUCTURE_1byp", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CUB:Cu+Binding+Site'>CUB</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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{{STRUCTURE_1byp| PDB=1byp | SCENE= }}
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|RELATEDENTRY=[[1byo|1BYO]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1byp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1byp OCA], [http://www.ebi.ac.uk/pdbsum/1byp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1byp RCSB]</span>
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}}
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'''E43K,D44K DOUBLE MUTANT PLASTOCYANIN FROM SILENE'''
'''E43K,D44K DOUBLE MUTANT PLASTOCYANIN FROM SILENE'''
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[[Category: Sugawara, H.]]
[[Category: Sugawara, H.]]
[[Category: Takabe, T.]]
[[Category: Takabe, T.]]
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[[Category: acidic patch]]
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[[Category: Acidic patch]]
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[[Category: double mutant]]
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[[Category: Double mutant]]
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[[Category: electron transfer]]
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[[Category: Electron transfer]]
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[[Category: photosynthesis]]
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[[Category: Photosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:07:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:11:01 2008''
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Revision as of 09:07, 2 May 2008

Template:STRUCTURE 1byp

E43K,D44K DOUBLE MUTANT PLASTOCYANIN FROM SILENE


Overview

Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation.

About this Structure

1BYP is a Single protein structure of sequence from Silene latifolia subsp. alba. Full crystallographic information is available from OCA.

Reference

Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene., Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y, J Biochem. 1999 May;125(5):899-903. PMID:10220581 Page seeded by OCA on Fri May 2 12:07:40 2008

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