1c12
From Proteopedia
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'''INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE''' | '''INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C12 OCA]. | |
==Reference== | ==Reference== | ||
Insight into odorant perception: the crystal structure and binding characteristics of antibody fragments directed against the musk odorant traseolide., Langedijk AC, Spinelli S, Anguille C, Hermans P, Nederlof J, Butenandt J, Honegger A, Cambillau C, Pluckthun A, J Mol Biol. 1999 Oct 1;292(4):855-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10525411 10525411] | Insight into odorant perception: the crystal structure and binding characteristics of antibody fragments directed against the musk odorant traseolide., Langedijk AC, Spinelli S, Anguille C, Hermans P, Nederlof J, Butenandt J, Honegger A, Cambillau C, Pluckthun A, J Mol Biol. 1999 Oct 1;292(4):855-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10525411 10525411] | ||
| - | [[Category: Mus musculus]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Anguille, C.]] | [[Category: Anguille, C.]] | ||
[[Category: Butenandt, J.]] | [[Category: Butenandt, J.]] | ||
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[[Category: Pluckthun, A.]] | [[Category: Pluckthun, A.]] | ||
[[Category: Spinelli, S.]] | [[Category: Spinelli, S.]] | ||
| - | [[Category: | + | [[Category: Antibody-antigen complex]] |
| - | [[Category: | + | [[Category: Cdrh3]] |
| - | [[Category: | + | [[Category: Immune system]] |
| - | [[Category: | + | [[Category: Musk odorant]] |
| - | [[Category: | + | [[Category: Odorant specificity]] |
| - | [[Category: | + | [[Category: Scfv fragment]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:12:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:12, 2 May 2008
INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE
Overview
Monoclonal antibodies were elicited against the small hydrophobic hapten traseolide, a commercially available musk fragrance. Antibody variable region sequences were found to belong to different sequence groups, and the binding characteristics of the corresponding antibody fragments were investigated. The antibodies M02/01/01 and M02/05/01 are highly homologous and differ in the binding pocket only at position H93. M02/05/01 (H93 Val) binds the hapten traseolide about 75-fold better than M02/01/01 (H93 Ala). A traseolide analog, missing only one methyl group, does not have the characteristic musk odorant fragrance. The antibody M02/05/01 binds this hapten analog about tenfold less tightly than the original traseolide hapten, and mimics the odorant receptor in this respect, while the antibody M02/01/01 does not distinguish between the analog and traseolide. To elucidate the structural basis for the fine specificity of binding, we determined the crystal structure of the Fab fragment of M02/05/01 complexed with the hapten at 2.6 A resolution. The crystal structure showed that only van der Waals interactions are involved in binding. The somatic Ala H93 Val mutation in M02/05/01 fills up an empty cavity in the binding pocket. This leads to an increase in binding energy and to the ability to discriminate between the hapten traseolide and its derivatives. The structural understanding of odorant specificity in an antibody gives insight in the physical principles on how specificity for such hydrophobic molecules may be achieved.
About this Structure
Full crystallographic information is available from OCA.
Reference
Insight into odorant perception: the crystal structure and binding characteristics of antibody fragments directed against the musk odorant traseolide., Langedijk AC, Spinelli S, Anguille C, Hermans P, Nederlof J, Butenandt J, Honegger A, Cambillau C, Pluckthun A, J Mol Biol. 1999 Oct 1;292(4):855-69. PMID:10525411 Page seeded by OCA on Fri May 2 12:12:20 2008
