1i9f
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(New page: 200px<br /> <applet load="1i9f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9f" /> '''STRUCTURAL CHARACTERIZATION OF THE COMPLEX ...)
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Revision as of 12:03, 8 November 2007
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STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE
Overview
INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the, Rev response element RNA, as the natural Rev peptide does. The RSG-1.2, sequence has features incompatible with the helical structure of the bound, Rev peptide, indicating that it must bind in a different conformation., RESULTS: The binding of the RSG-1.2 peptide to the Rev response element, RNA was characterized using multinuclear, multidimensional NMR. The, RSG-1.2 peptide is shown to bind with the N-terminal segment of the, peptide along the major groove in an extended conformation and turn, preceding a C-terminal helical segment, which crosses the RNA groove in, the region widened by the presence of purine-purine base pairs. These, features make the details of the bound state rather different than that of, the Rev peptide which targets the same RNA sequence binding as a single, helix along the groove axis. CONCLUSIONS: These studies further, demonstrate the versatility of arginine-rich peptides in recognition of, specific RNA elements and the lack of conserved structural features in the, bound state.
About this Structure
1I9F is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:11358697
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