2xhl

From Proteopedia

(Difference between revisions)

Revision as of 15:14, 8 July 2020

Structure of a functional derivative of Clostridium botulinum neurotoxin type B

Structural highlights

2xhl is a 2 chain structure with sequence from "bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1s0d, 1epw, 1g9b, 1f82, 2etf, 1i1e, 1s0f, 1g9a, 1g9c, 1s0b, 1s0g, 1s0c, 1g9d, 1z0h, 1f31, 1s0e
Activity:	Bontoxilysin, with EC number 3.4.24.69
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BXB_CLOBO] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2.

Publication Abstract from PubMed

Botulinum neurotoxins (BoNTs) cause flaccid paralysis by inhibiting neurotransmission at cholinergic nerve terminals. BoNTs consist of three essential domains for toxicity: the cell binding domain (Hc), the translocation domain (Hn) and the catalytic domain (LC). A functional derivative (LHn) of the parent neurotoxin B composed of Hn and LC domains was recombinantly produced and characterised. LHn/B crystallographic structure at 2.8A resolution is reported. The catalytic activity of LHn/B towards recombinant human VAMP was analysed by substrate cleavage assay and showed a higher specificity for VAMP-1, -2 compared to VAMP-3. LHn/B also showed measurable activity in living spinal cord neurons. Despite lacking the Hc (cell-targeting) domain, LHn/B retained the capacity to internalize and cleave intracellular VAMP-1 and -2 when added to the cells at high concentration. These activities of the LHn/B fragment demonstrate the utility of engineered botulinum neurotoxin fragments as analytical tools to study the mechanisms of action of BoNT neurotoxins and of SNARE proteins.

Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B.,Masuyer G, Beard M, Cadd VA, Chaddock JA, Acharya KR J Struct Biol. 2010 Nov 13. PMID:21078393^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Masuyer G, Beard M, Cadd VA, Chaddock JA, Acharya KR. Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B. J Struct Biol. 2010 Nov 13. PMID:21078393 doi:10.1016/j.jsb.2010.11.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xhl"

@@ Line 1: / Line 1: @@
 ==Structure of a functional derivative of Clostridium botulinum neurotoxin type B==
-<StructureSection load='2xhl' size='340' side='right' caption='[[2xhl]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='2xhl' size='340' side='right'caption='[[2xhl]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xhl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XHL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xhl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XHL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1s0d|1s0d]], [[1epw|1epw]], [[1g9b|1g9b]], [[1f82|1f82]], [[2etf|2etf]], [[1i1e|1i1e]], [[1s0f|1s0f]], [[1g9a|1g9a]], [[1g9c|1g9c]], [[1s0b|1s0b]], [[1s0g|1s0g]], [[1s0c|1s0c]], [[1g9d|1g9d]], [[1z0h|1z0h]], [[1f31|1f31]], [[1s0e|1s0e]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhl OCA], [http://pdbe.org/2xhl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xhl RCSB], [http://www.ebi.ac.uk/pdbsum/2xhl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhl ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhl OCA], [http://pdbe.org/2xhl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xhl RCSB], [http://www.ebi.ac.uk/pdbsum/2xhl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhl ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 ==See Also==
-*[[Botulinum neurotoxin|Botulinum neurotoxin]]
+*[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]]
 == References ==
 <references/>
@@ Line 29: / Line 29: @@
 [[Category: Bacillus botulinus van ermengem 1896]]
 [[Category: Bontoxilysin]]
+[[Category: Large Structures]]
 [[Category: Acharya, K R]]
 [[Category: Beard, M]]

2xhl

From Proteopedia

Revision as of 15:14, 8 July 2020

Structure of a functional derivative of Clostridium botulinum neurotoxin type B

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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