6v20
From Proteopedia
(Difference between revisions)
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<SX load='6v20' size='340' side='right' viewer='molstar' caption='[[6v20]], [[Resolution|resolution]] 2.13Å' scene=''> | <SX load='6v20' size='340' side='right' viewer='molstar' caption='[[6v20]], [[Resolution|resolution]] 2.13Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6v20]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V20 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V20 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v20]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V20 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V20 FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v20 OCA], [http://pdbe.org/6v20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v20 RCSB], [http://www.ebi.ac.uk/pdbsum/6v20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v20 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v20 OCA], [http://pdbe.org/6v20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v20 RCSB], [http://www.ebi.ac.uk/pdbsum/6v20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v20 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref> | [[http://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although the advent of direct electron detectors (DEDs) and software developments have enabled the routine use of single-particle cryogenic electron microscopy (cryo-EM) for structure determination of well-behaved specimens to high-resolution, there nonetheless remains a discrepancy between the resolutions attained for biological specimens and the information limits of modern transmission electron microscopes (TEMs). Instruments operating at 300kV equipped with DEDs are the current paradigm for high-resolution single-particle cryo-EM, while 200kV TEMs remain comparatively underutilized for purposes beyond sample screening. Here, we expand upon our prior work and demonstrate that one such 200kV microscope, the Talos Arctica, equipped with a K2 DED is capable of determining structures of macromolecules to as high as approximately 1.7A resolution. At this resolution, ordered water molecules are readily assigned and holes in aromatic residues can be clearly distinguished in the reconstructions. This work emphasizes the utility of 200kV electrons for high-resolution single-particle cryo-EM and applications such as structure-based drug design. | ||
| + | |||
| + | Sub-2 Angstrom resolution structure determination using single-particle cryo-EM at 200keV.,Wu M, Lander GC, Herzik MA Jr J Struct Biol X. 2020 Feb 28;4:100020. doi: 10.1016/j.yjsbx.2020.100020., eCollection 2020. PMID:32647824<ref>PMID:32647824</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6v20" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Aldolase 3D structures|Aldolase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</SX> | </SX> | ||
| + | [[Category: European rabbit]] | ||
[[Category: Fructose-bisphosphate aldolase]] | [[Category: Fructose-bisphosphate aldolase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
Revision as of 11:38, 22 July 2020
Rabbit muscle aldolase determined using single-particle cryo-EM at 200 keV
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