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2xz7

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Revision as of 12:27, 22 July 2020

CRYSTAL STRUCTURE OF THE PHOSPHOENOLPYRUVATE-BINDING DOMAIN OF ENZYME I IN COMPLEX WITH PHOSPHOENOLPYRUVATE FROM THE THERMOANAEROBACTER TENGCONGENSIS PEP-SUGAR PHOSPHOTRANSFERASE SYSTEM (PTS)

Structural highlights

2xz7 is a 1 chain structure with sequence from As 1.2430. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2xz9, 2bg5
Activity:	Phosphoenolpyruvate--protein phosphotransferase, with EC number 2.7.3.9
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q8R7R4_THETN] General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).[PIRNR:PIRNR000732]

Publication Abstract from PubMed

Thermoanaerobacter tengcongensis is a thermophilic eubacterium that has a phosphoenolpyruvate (PEP) sugar phosphotransferase system (PTS) of 22 proteins. The general PTS proteins, enzyme I and HPr, and the transporters for N-acetylglucosamine (EIICB(GlcNAc)) and fructose (EIIBC(Fru)) have thermal unfolding transitions at approximately 90 degrees C and a temperature optimum for in vitro sugar phosphotransferase activity of 65 degrees C. The phosphocysteine of a EIICB(GlcNAc) mutant is unusually stable at room temperature with a t(1/2) of 60 h. The PEP binding C-terminal domain of enzyme I (EIC) forms a metastable covalent adduct with PEP at 65 degrees C. Crystallization of this adduct afforded the 1.68 A resolution structure of EIC with a molecule of pyruvate in the active site. We also report the 1.83 A crystal structure of the EIC-PEP complex. The comparison of the two structures with the apo form and with full-length EI shows differences between the active site side chain conformations of the PEP and pyruvate states but not between the pyruvate and apo states. In the presence of PEP, Arg465 forms a salt bridge with the phosphate moiety while Glu504 forms salt bridges with Arg186 and Arg195 of the N-terminal domain of enzyme I (EIN), which stabilizes a conformation appropriate for the in-line transfer of the phosphoryl moiety from PEP to His191. After transfer, Arg465 swings 4.8 A away to form an alternative salt bridge with the carboxylate of Glu504. Glu504 loses the grip of Arg186 and Arg195, and the EIN domain can swing away to hand on the phosphoryl group to the phosphoryl carrier protein HPr.

Phosphoenolpyruvate: sugar phosphotransferase system from the hyperthermophilic Thermoanaerobacter tengcongensis.,Navdaeva V, Zurbriggen A, Waltersperger S, Schneider P, Oberholzer AE, Bahler P, Bachler C, Grieder A, Baumann U, Erni B Biochemistry. 2011 Feb 22;50(7):1184-93. Epub 2011 Jan 20. PMID:21250658^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Navdaeva V, Zurbriggen A, Waltersperger S, Schneider P, Oberholzer AE, Bahler P, Bachler C, Grieder A, Baumann U, Erni B. Phosphoenolpyruvate: sugar phosphotransferase system from the hyperthermophilic Thermoanaerobacter tengcongensis. Biochemistry. 2011 Feb 22;50(7):1184-93. Epub 2011 Jan 20. PMID:21250658 doi:10.1021/bi101721f

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xz7"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF THE PHOSPHOENOLPYRUVATE-BINDING DOMAIN OF ENZYME I IN COMPLEX WITH PHOSPHOENOLPYRUVATE FROM THE THERMOANAEROBACTER TENGCONGENSIS PEP-SUGAR PHOSPHOTRANSFERASE SYSTEM (PTS)==
-<StructureSection load='2xz7' size='340' side='right' caption='[[2xz7]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
+<StructureSection load='2xz7' size='340' side='right'caption='[[2xz7]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xz7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_1.2430 As 1.2430]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZ7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xz7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_1.2430 As 1.2430]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZ7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XZ7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xz9|2xz9]], [[2bg5|2bg5]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate--protein_phosphotransferase Phosphoenolpyruvate--protein phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.9 2.7.3.9] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xz7 OCA], [http://pdbe.org/2xz7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xz7 RCSB], [http://www.ebi.ac.uk/pdbsum/2xz7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xz7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xz7 OCA], [http://pdbe.org/2xz7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xz7 RCSB], [http://www.ebi.ac.uk/pdbsum/2xz7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xz7 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 ==See Also==
-*[[Phosphotransferase|Phosphotransferase]]
+*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
 == References ==
 <references/>
@@ Line 28: / Line 28: @@
 </StructureSection>
 [[Category: As 1 2430]]
+[[Category: Large Structures]]
 [[Category: Phosphoenolpyruvate--protein phosphotransferase]]
 [[Category: Baumann, U]]

2xz7

From Proteopedia

Revision as of 12:27, 22 July 2020

CRYSTAL STRUCTURE OF THE PHOSPHOENOLPYRUVATE-BINDING DOMAIN OF ENZYME I IN COMPLEX WITH PHOSPHOENOLPYRUVATE FROM THE THERMOANAEROBACTER TENGCONGENSIS PEP-SUGAR PHOSPHOTRANSFERASE SYSTEM (PTS)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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