2y5y

From Proteopedia

(Difference between revisions)

Revision as of 12:36, 22 July 2020

Crystal structure of LacY in complex with an affinity inactivator

Structural highlights

2y5y is a 2 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1pv6, 2v8n, 1pv7, 2cfp, 2cfq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).

Publication Abstract from PubMed

Lactose permease of Escherichia coli (LacY) with a single-Cys residue in place of A122 (helix IV) transports galactopyranosides and is specifically inactivated by methanethiosulfonyl-galactopyranosides (MTS-gal), which behave as unique suicide substrates. In order to study the mechanism of inactivation more precisely, we solved the structure of single-Cys122 LacY in complex with covalently bound MTS-gal. This structure exhibits an inward-facing conformation similar to that observed previously with a slight narrowing of the cytoplasmic cavity. MTS-gal is bound covalently, forming a disulfide bond with C122 and positioned between R144 and W151. E269, a residue essential for binding, coordinates the C-4 hydroxyl of the galactopyranoside moiety. The location of the sugar is in accord with many biochemical studies.

Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.,Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. Epub 2011 May 18. PMID:21593407^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J. Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition. Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. Epub 2011 May 18. PMID:21593407 doi:10.1073/pnas.1105687108

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y5y"

@@ Line 1: / Line 1: @@
 ==Crystal structure of LacY in complex with an affinity inactivator==
-<StructureSection load='2y5y' size='340' side='right' caption='[[2y5y]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
+<StructureSection load='2y5y' size='340' side='right'caption='[[2y5y]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y5y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y5Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y5y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2Y5Y FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=TGA:METHANETHIOSULFONYL-GALACTOSIDE'>TGA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=TGA:METHANETHIOSULFONYL-GALACTOSIDE'>TGA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pv6|1pv6]], [[2v8n|2v8n]], [[1pv7|1pv7]], [[2cfp|2cfp]], [[2cfq|2cfq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y5y OCA], [http://pdbe.org/2y5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y5y RCSB], [http://www.ebi.ac.uk/pdbsum/2y5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y5y ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2y5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y5y OCA], [http://pdbe.org/2y5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y5y RCSB], [http://www.ebi.ac.uk/pdbsum/2y5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y5y ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Ecoli]]
+[[Category: Large Structures]]
 [[Category: Abramson, J]]
 [[Category: Chaptal, V]]

2y5y

From Proteopedia

Revision as of 12:36, 22 July 2020

Crystal structure of LacY in complex with an affinity inactivator

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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