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2yjy

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Revision as of 12:54, 22 July 2020

A specific and modular binding code for cytosine recognition in PUF domains

Structural highlights

2yjy is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1ib2, 1m8y, 1m8z, 1m8w, 1m8x
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PUM1_HUMAN] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).

Publication Abstract from PubMed

Pumilio/fem-3 mRNA-binding factor (PUF) proteins possess a recognition code for bases A, U, and G, allowing designed RNA sequence specificity of their modular Pumilio (PUM) repeats. However, recognition side chains in a PUM repeat for cytosine are unknown. Here we report identification of a cytosine-recognition code by screening random amino acid combinations at conserved RNA recognition positions using a yeast three-hybrid system. This C-recognition code is specific and modular as specificity can be transferred to different positions in the RNA recognition sequence. A crystal structure of a modified PUF domain reveals specific contacts between an arginine side chain and the cytosine base. We applied the C-recognition code to design PUF domains that recognize targets with multiple cytosines and to generate engineered splicing factors that modulate alternative splicing. Finally, we identified a divergent yeast PUF protein, Nop9p, that may recognize natural target RNAs with cytosine. This work deepens our understanding of natural PUF protein target recognition and expands the ability to engineer PUF domains to recognize any RNA sequence.

Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains.,Dong S, Wang Y, Cassidy-Amstutz C, Lu G, Bigler R, Jezyk MR, Li C, Hall TM, Wang Z J Biol Chem. 2011 Jul 29;286(30):26732-42. Epub 2011 Jun 8. PMID:21653694^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dong S, Wang Y, Cassidy-Amstutz C, Lu G, Bigler R, Jezyk MR, Li C, Hall TM, Wang Z. Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains. J Biol Chem. 2011 Jul 29;286(30):26732-42. Epub 2011 Jun 8. PMID:21653694 doi:10.1074/jbc.M111.244889

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yjy"

@@ Line 1: / Line 1: @@
 ==A specific and modular binding code for cytosine recognition in PUF domains==
-<StructureSection load='2yjy' size='340' side='right' caption='[[2yjy]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='2yjy' size='340' side='right'caption='[[2yjy]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2yjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2yjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YJY FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ib2|1ib2]], [[1m8y|1m8y]], [[1m8z|1m8z]], [[1m8w|1m8w]], [[1m8x|1m8x]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjy OCA], [http://pdbe.org/2yjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yjy RCSB], [http://www.ebi.ac.uk/pdbsum/2yjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2yjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjy OCA], [http://pdbe.org/2yjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yjy RCSB], [http://www.ebi.ac.uk/pdbsum/2yjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjy ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Bigler, R]]
 [[Category: Cassidy-Amstutz, C]]

2yjy

From Proteopedia

Revision as of 12:54, 22 July 2020

A specific and modular binding code for cytosine recognition in PUF domains

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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