1c9u

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[[Image:1c9u.gif|left|200px]]
[[Image:1c9u.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1c9u |SIZE=350|CAPTION= <scene name='initialview01'>1c9u</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1c9u", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Quinoprotein_glucose_dehydrogenase Quinoprotein glucose dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.5.2 1.1.5.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1c9u| PDB=1c9u | SCENE= }}
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|RELATEDENTRY=[[1qbi|1QBI]], [[1cq1|1CQ1]], [[1cru|1CRU]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9u OCA], [http://www.ebi.ac.uk/pdbsum/1c9u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1c9u RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ'''
'''CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ'''
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[[Category: Oubrie, A.]]
[[Category: Oubrie, A.]]
[[Category: Rozeboom, H J.]]
[[Category: Rozeboom, H J.]]
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[[Category: beta-propeller]]
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[[Category: Beta-propeller]]
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[[Category: cofactor binding]]
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[[Category: Cofactor binding]]
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[[Category: superbarrel]]
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[[Category: Superbarrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:30:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:17:38 2008''
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Revision as of 09:30, 2 May 2008

Image:1c9u.gif

Template:STRUCTURE 1c9u

CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ


Overview

Soluble glucose dehydrogenase (s-GDH; EC 1.1.99.17) is a classical quinoprotein which requires the cofactor pyrroloquinoline quinone (PQQ) to oxidize glucose to gluconolactone. The reaction mechanism of PQQ-dependent enzymes has remained controversial due to the absence of comprehensive structural data. We have determined the X-ray structure of s-GDH with the cofactor at 2.2 A resolution, and of a complex with reduced PQQ and glucose at 1.9 A resolution. These structures reveal the active site of s-GDH, and show for the first time how a functionally bound substrate interacts with the cofactor in a PQQ-dependent enzyme. Twenty years after the discovery of PQQ, our results finally provide conclusive evidence for a reaction mechanism comprising general base-catalyzed hydride transfer, rather than the generally accepted covalent addition-elimination mechanism. Thus, PQQ-dependent enzymes use a mechanism similar to that of nicotinamide- and flavin-dependent oxidoreductases.

About this Structure

1C9U is a Single protein structure of sequence from Acinetobacter calcoaceticus. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of soluble quinoprotein glucose dehydrogenase., Oubrie A, Rozeboom HJ, Kalk KH, Olsthoorn AJ, Duine JA, Dijkstra BW, EMBO J. 1999 Oct 1;18(19):5187-94. PMID:10508152 Page seeded by OCA on Fri May 2 12:30:24 2008

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