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2zyk

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Revision as of 12:11, 29 July 2020

Crystal structure of cyclo/maltodextrin-binding protein complexed with gamma-cyclodextrin

Structural highlights

2zyk is a 4 chain structure with sequence from Atcc 43649. This structure supersedes the now removed PDB entry 2dfz. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of a Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein (TvuCMBP) complexed with gamma-cyclodextrin has been determined. Like Escherichia coli maltodextrin-binding protein (EcoMBP) and other bacterial sugar-binding proteins, TvuCMBP consists of two domains, an N- and a C-domain, both of which are composed of a central beta-sheet surrounded by alpha-helices; the domains are joined by a hinge region containing three segments. gamma-Cyclodextrin is located at a cleft formed by the two domains. A common functional conformational change has been reported in this protein family, which involves switching from an open form to a sugar-transporter bindable form, designated a closed form. The TvuCMBP-gamma-cyclodextrin complex structurally resembles the closed form of EcoMBP, indicating that TvuCMBP complexed with gamma-cyclodextrin adopts the closed form. The fluorescence measurements also showed that the affinities of TvuCMBP for cyclodextrins were almost equal to those for maltooligosaccharides. Despite having similar folds, the sugar-binding site of the N-domain part of TvuCMBP and other bacterial sugar-binding proteins are strikingly different. In TvuCMBP, the side-chain of Leu59 protrudes from the N-domain part into the sugar-binding cleft and orients toward the central cavity of gamma-cyclodextrin, thus Leu59 appears to play the key role in binding. The cleft of the sugar-binding site of TvuCMBP is also wider than that of EcoMBP. These findings suggest that the sugar-binding site of the N-domain part and the wide cleft are critical in determining the specificity of TvuCMBP for gamma-cyclodextrin.

Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein.,Tonozuka T, Sogawa A, Yamada M, Matsumoto N, Yoshida H, Kamitori S, Ichikawa K, Mizuno M, Nishikawa A, Sakano Y FEBS J. 2007 Apr;274(8):2109-20. Epub 2007 Mar 20. PMID:17371546^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tonozuka T, Sogawa A, Yamada M, Matsumoto N, Yoshida H, Kamitori S, Ichikawa K, Mizuno M, Nishikawa A, Sakano Y. Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein. FEBS J. 2007 Apr;274(8):2109-20. Epub 2007 Mar 20. PMID:17371546 doi:http://dx.doi.org/10.1111/j.1742-4658.2007.05753.x

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2zyk"

@@ Line 1: / Line 1: @@
 ==Crystal structure of cyclo/maltodextrin-binding protein complexed with gamma-cyclodextrin==
-<StructureSection load='2zyk' size='340' side='right' caption='[[2zyk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='2zyk' size='340' side='right'caption='[[2zyk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2zyk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43649 Atcc 43649]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2dfz 2dfz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZYK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2zyk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43649 Atcc 43649]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2dfz 2dfz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ZYK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyk OCA], [http://pdbe.org/2zyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zyk RCSB], [http://www.ebi.ac.uk/pdbsum/2zyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyk ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2zyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyk OCA], [http://pdbe.org/2zyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zyk RCSB], [http://www.ebi.ac.uk/pdbsum/2zyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyk ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Atcc 43649]]
+[[Category: Large Structures]]
 [[Category: Ichikawa, K]]
 [[Category: Kamitori, S]]

2zyk

From Proteopedia

Revision as of 12:11, 29 July 2020

Crystal structure of cyclo/maltodextrin-binding protein complexed with gamma-cyclodextrin

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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