6vk2
From Proteopedia
(Difference between revisions)
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==NMR solution structure of Grb2-SH2 domain at pH 7== | ==NMR solution structure of Grb2-SH2 domain at pH 7== | ||
| - | <StructureSection load='6vk2' size='340' side='right'caption='[[6vk2]]' scene=''> | + | <StructureSection load='6vk2' size='340' side='right'caption='[[6vk2]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[6vk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VK2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VK2 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vk2 OCA], [http://pdbe.org/6vk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vk2 RCSB], [http://www.ebi.ac.uk/pdbsum/6vk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vk2 ProSAT]</span></td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bmb|1bmb]], [[1bm2|1bm2]], [[1fhs|1fhs]], [[1jyq|1jyq]], [[1jyr|1jyr]], [[1jyu|1jyu]], [[1qg1|1qg1]], [[1tze|1tze]], [[1zfp|1zfp]], [[2aoa|2aoa]], [[2h46|2h46]], [[2h5k|2h5k]], [[2how|2how]], [[3c7i|3c7i]], [[3imd|3imd]], [[3imj|3imj]], [[3in7|3in7]], [[3kfj|3kfj]], [[3mxc|3mxc]], [[3n7y|3n7y]], [[3n84|3n84]], [[3n8m|3n8m]], [[3ov1|3ov1]], [[3s8l|3s8l]], [[3wa4|3wa4]]</td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRB2, ASH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vk2 OCA], [http://pdbe.org/6vk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vk2 RCSB], [http://www.ebi.ac.uk/pdbsum/6vk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vk2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GRB2_HUMAN GRB2_HUMAN]] Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.<ref>PMID:1322798</ref> <ref>PMID:8178156</ref> <ref>PMID:19815557</ref> Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.<ref>PMID:1322798</ref> <ref>PMID:8178156</ref> <ref>PMID:19815557</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein composed of three domains, an N-terminal SH3 (nSH3), SH2 and a C-terminal SH3 (cSH3) domains. This multi-domain protein has been reported to be a key factor in many signaling pathways related to controlling cell survival, differentiation, and growth. The Grb2-SH2 domain has been a focus for the study of the interaction with peptides and small molecules to act as inhibitors in uncontrolled cell growth, and consequently inhibit tumor proliferation. Here we describe the almost complete assignment of the free SH2 domain at pH 7. This work prepares the ground for further structural studies, backbone dynamics, mapping of interactions and drug screening and development. TalosN secondary structure prediction showed great similarity with the available structures in the PDB. | ||
| + | |||
| + | NMR assignment of free (1)H, (15)N and (13)C-Grb2-SH2 domain.,Sanches K, Caruso IP, Almeida FCL, Melo FA Biomol NMR Assign. 2019 Oct;13(2):295-298. doi: 10.1007/s12104-019-09894-x. Epub , 2019 Apr 26. PMID:31028611<ref>PMID:31028611</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6vk2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Almeida | + | [[Category: Almeida, F C.L]] |
| - | [[Category: Caruso | + | [[Category: Caruso, I P]] |
| - | [[Category: Melo | + | [[Category: Melo, F A]] |
| - | [[Category: Sanches K]] | + | [[Category: Sanches, K]] |
| + | [[Category: Cell cycle]] | ||
| + | [[Category: Dynamic]] | ||
| + | [[Category: Grb2]] | ||
| + | [[Category: Sh2 domain]] | ||
Revision as of 05:57, 5 August 2020
NMR solution structure of Grb2-SH2 domain at pH 7
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Categories: Human | Large Structures | Almeida, F C.L | Caruso, I P | Melo, F A | Sanches, K | Cell cycle | Dynamic | Grb2 | Sh2 domain
