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4uht

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Revision as of 06:23, 5 August 2020

Crystal structure of the DNA binding domain of CpxR from E. coli

Structural highlights

4uht is a 2 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4uhj, 4uhk, 4uhs
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CPXR_ECOLI] Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).^[1] ^[2] ^[3]

Publication Abstract from PubMed

The transcriptional regulator CpxR mediates an adaptive response to envelope stress, tightly linked to virulence and antibiotics resistance in several Gammaproteobacteria pathogens. In this work, we integrated crystallographic and small-angle X-ray scattering data to gain insights into the structure and conformational plasticity of CpxR from Escherichia coli. CpxR dimerizes through two alternative interaction surfaces. Moreover, widely different CpxR conformations coexist in solution, from compact to fully extended ones. The possible functional implications of these structural features are discussed.

Conformational plasticity of the response regulator CpxR, a key player in Gammaproteobacteria virulence and drug-resistance.,Mechaly AE, Haouz A, Sassoon N, Buschiazzo A, Betton JM, Alzari PM J Struct Biol. 2018 Aug 4. pii: S1047-8477(18)30196-5. doi:, 10.1016/j.jsb.2018.08.001. PMID:30086390^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dorsey-Oresto A, Lu T, Mosel M, Wang X, Salz T, Drlica K, Zhao X. YihE kinase is a central regulator of programmed cell death in bacteria. Cell Rep. 2013 Feb 21;3(2):528-37. doi: 10.1016/j.celrep.2013.01.026. Epub 2013, Feb 14. PMID:23416055 doi:http://dx.doi.org/10.1016/j.celrep.2013.01.026
↑ Danese PN, Snyder WB, Cosma CL, Davis LJ, Silhavy TJ. The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP. Genes Dev. 1995 Feb 15;9(4):387-98. PMID:7883164
↑ Raivio TL, Silhavy TJ. Transduction of envelope stress in Escherichia coli by the Cpx two-component system. J Bacteriol. 1997 Dec;179(24):7724-33. PMID:9401031
↑ Mechaly AE, Haouz A, Sassoon N, Buschiazzo A, Betton JM, Alzari PM. Conformational plasticity of the response regulator CpxR, a key player in Gammaproteobacteria virulence and drug-resistance. J Struct Biol. 2018 Aug 4. pii: S1047-8477(18)30196-5. doi:, 10.1016/j.jsb.2018.08.001. PMID:30086390 doi:http://dx.doi.org/10.1016/j.jsb.2018.08.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4uht"

Categories: Ecoli | Large Structures | Alzari, P M.A | Mechaly, A E | Transcription

@@ Line 1: / Line 1: @@
 ==Crystal structure of the DNA binding domain of CpxR from E. coli==
-<StructureSection load='4uht' size='340' side='right' caption='[[4uht]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
+<StructureSection load='4uht' size='340' side='right'caption='[[4uht]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4uht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4uht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4UHT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uhj|4uhj]], [[4uhk|4uhk]], [[4uhs|4uhs]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uht OCA], [http://pdbe.org/4uht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uht RCSB], [http://www.ebi.ac.uk/pdbsum/4uht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uht ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4uht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uht OCA], [http://pdbe.org/4uht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uht RCSB], [http://www.ebi.ac.uk/pdbsum/4uht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uht ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Ecoli]]
+[[Category: Large Structures]]
 [[Category: Alzari, P M.A]]
 [[Category: Mechaly, A E]]
 [[Category: Transcription]]

4uht

From Proteopedia

Revision as of 06:23, 5 August 2020

Crystal structure of the DNA binding domain of CpxR from E. coli

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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