1ccj

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[[Image:1ccj.jpg|left|200px]]
[[Image:1ccj.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ccj |SIZE=350|CAPTION= <scene name='initialview01'>1ccj</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1ccj", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=ACT:Removal+Of+PHE+202+Forms+An+Internal+Cavity+Adjacent+To+...'>ACT</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= CCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_1ccj| PDB=1ccj | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ccj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ccj OCA], [http://www.ebi.ac.uk/pdbsum/1ccj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ccj RCSB]</span>
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}}
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'''CONFORMER SELECTION BY LIGAND BINDING OBSERVED WITH PROTEIN CRYSTALLOGRAPHY'''
'''CONFORMER SELECTION BY LIGAND BINDING OBSERVED WITH PROTEIN CRYSTALLOGRAPHY'''
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[[Category: Musah, R A.]]
[[Category: Musah, R A.]]
[[Category: Wilcox, S K.]]
[[Category: Wilcox, S K.]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: peroxidase]]
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[[Category: Peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:35:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:19:01 2008''
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Revision as of 09:35, 2 May 2008

Image:1ccj.jpg

Template:STRUCTURE 1ccj

CONFORMER SELECTION BY LIGAND BINDING OBSERVED WITH PROTEIN CRYSTALLOGRAPHY


Overview

A large-scale movement between "closed" and "open" conformations of a protein loop was observed directly with protein crystallography by trapping individual conformers through binding of an exogenous ligand and characterization with solution kinetics. The buried indole ring of Trp191 in cytochrome c peroxidase (CCP) was displaced by exogenous ligands, causing a conformational change of loop Pro190-Asn195 and exposing Trp191 to the protein surface. Kinetic measurements are consistent with a two-step binding mechanism in which the rate-limiting step is a transition of the protein to the open state, which then binds the ligand. This large-scale conformational change of a functionally important region of CCP is independent of ligand and indicates that about 4% of the wild-type protein is in the open form in solution at any given time.

About this Structure

1CCJ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Protein conformer selection by ligand binding observed with crystallography., Cao Y, Musah RA, Wilcox SK, Goodin DB, McRee DE, Protein Sci. 1998 Jan;7(1):72-8. PMID:9514261 Page seeded by OCA on Fri May 2 12:35:00 2008

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