1m05
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1m05" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m05, resolution 1.9Å" /> '''HLA B8 in complex wi...)
Next diff →
Revision as of 12:12, 8 November 2007
|
HLA B8 in complex with an Epstein Barr Virus determinant
Overview
EBV is a ubiquitous human pathogen that chronically infects up to 90% of, the population. Persistent viral infection is characterized by latency and, periods of viral replication that are kept in check by a strong antiviral, CTL response. Despite the size of the EBV genome, CTL immunity focuses on, only a few viral determinants but expands a large primary and memory, response toward these epitopes. In unrelated HLA-B8(+) individuals, the, response to the immunodominant latent Ag FLRGRAYGL from Epstein Barr, nuclear Ag 3A is largely comprised of CTL clones with identical conserved, alphabeta TCR structures. To better understand the structural correlates, of Ag immunodominance and TCR selection bias, we have solved the crystal, structure of the HLA-B8-FLRGRAYGL peptide complex to a resolution of 1.9, A. The structure confirms the importance of P3-Arg, P5-Arg, and P9-Leu as, dominant anchor residues involved in peptide binding to HLA-B8. A bulged, conformation of the bound peptide provides a structural basis for the, critical role of the P7-Tyr residue in T cell recognition. The peptide, also induces backbone and side-chain conformational changes in HLA-B8 that, are transmitted along the peptide-binding groove in a domino effect. The, HLA-B8-FLRGRAYGL complex crystallizes as a dimer in the asymmetric unit, and is oriented such that both peptide ligands are projected in the same, plane suggesting a higher order arrangement of MHC-peptide complexes that, could be involved in formation of the class I Ag-loading complex or in T, cell activation.
About this Structure
1M05 is a Protein complex structure of sequences from Homo sapiens with CD as ligand. Full crystallographic information is available from OCA.
Reference
The structure of HLA-B8 complexed to an immunodominant viral determinant: peptide-induced conformational changes and a mode of MHC class I dimerization., Kjer-Nielsen L, Clements CS, Brooks AG, Purcell AW, Fontes MR, McCluskey J, Rossjohn J, J Immunol. 2002 Nov 1;169(9):5153-60. PMID:12391232
Page seeded by OCA on Thu Nov 8 14:18:15 2007
