6i9g
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i9g OCA], [http://pdbe.org/6i9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i9g RCSB], [http://www.ebi.ac.uk/pdbsum/6i9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i9g ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i9g OCA], [http://pdbe.org/6i9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i9g RCSB], [http://www.ebi.ac.uk/pdbsum/6i9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i9g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Using a newly discovered encapsulin from Mycolicibacterium hassiacum, several biocatalysts were packaged in this robust protein cage. The encapsulin was found to be easy to produce as recombinant protein. Elucidation of its crystal structure revealed that it is a spherical protein cage of 60 protomers (diameter of 23 nm) with narrow pores. By developing an effective coexpression and isolation procedure, the effect of packaging a variety of biocatalysts could be evaluated. It was shown that encapsulation results in a significantly higher stability of the biocatalysts. Most of the targeted cofactor-containing biocatalysts remained active in the encapsulin. Due to the restricted diameters of the encapsulin pores (5-9 A), the protein cage protects the encapsulated enzymes from bulky compounds. The work shows that encapsulins may be valuable tools to tune the properties of biocatalysts such as stability and substrate specificity. | ||
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| + | Structure of a robust bacterial protein cage and its application as a versatile biocatalytic platform through enzyme encapsulation.,Loncar N, Rozeboom HJ, Franken LE, Stuart MCA, Fraaije MW Biochem Biophys Res Commun. 2020 Aug 27;529(3):548-553. doi:, 10.1016/j.bbrc.2020.06.059. Epub 2020 Jul 15. PMID:32736672<ref>PMID:32736672</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6i9g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 09:54, 12 August 2020
Crystal structure of encapsulin from Mycolicibacterium hassiacum
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