6iww
From Proteopedia
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==Cryo-EM structure of the S. typhimurium oxaloacetate decarboxylase beta-gamma sub-complex== | ==Cryo-EM structure of the S. typhimurium oxaloacetate decarboxylase beta-gamma sub-complex== | ||
| - | <StructureSection load='6iww' size='340' side='right'caption='[[6iww]]' scene=''> | + | <StructureSection load='6iww' size='340' side='right'caption='[[6iww]], [[Resolution|resolution]] 3.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6IWW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6iww]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Salet Salet]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IWW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6IWW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6iww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iww OCA], [http://pdbe.org/6iww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iww RCSB], [http://www.ebi.ac.uk/pdbsum/6iww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iww ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxaloacetate_decarboxylase_(Na(+)_extruding) Oxaloacetate decarboxylase (Na(+) extruding)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.2.4.2 7.2.4.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6iww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iww OCA], [http://pdbe.org/6iww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iww RCSB], [http://www.ebi.ac.uk/pdbsum/6iww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iww ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/A0A0F7JAV8_SALTM A0A0F7JAV8_SALTM]] Catalyzes the decarboxylation of oxaloacetate coupled to Na(+) translocation.[PIRNR:PIRNR015658] [[http://www.uniprot.org/uniprot/A0A0D6I9X5_SALTM A0A0D6I9X5_SALTM]] Catalyzes the decarboxylation of oxaloacetate coupled to Na(+) translocation.[HAMAP-Rule:MF_00404][RuleBase:RU004278] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The oxaloacetate decarboxylase sodium pump (OAD) is a unique primary-active transporter that utilizes the free energy derived from oxaloacetate decarboxylation for sodium transport across the cell membrane. It is composed of 3 subunits: the alpha subunit catalyzes carboxyl-transfer from oxaloacetate to biotin, the membrane integrated beta subunit catalyzes the subsequent carboxyl-biotin decarboxylation and the coupled sodium transport, the gamma subunit interacts with the alpha and beta subunits and stabilizes the OAD complex. We present here structure of the Salmonella typhimurium OAD betagamma sub-complex. The structure revealed that the beta and gamma subunits form a beta3gamma3 hetero-hexamer with extensive interactions between the subunits and shed light on the OAD holo-enzyme assembly. Structure-guided functional studies provided insights into the sodium binding sites in the beta subunit and the coupling between carboxyl-biotin decarboxylation and sodium transport by the OAD beta subunit. | ||
| + | |||
| + | Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump.,Xu X, Shi H, Gong X, Chen P, Gao Y, Zhang X, Xiang S Elife. 2020 May 27;9. pii: 53853. doi: 10.7554/eLife.53853. PMID:32459174<ref>PMID:32459174</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6iww" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Shi H]] | + | [[Category: Salet]] |
| - | [[Category: Xiang S]] | + | [[Category: Shi, H]] |
| - | [[Category: Xu X]] | + | [[Category: Xiang, S]] |
| - | [[Category: Zhang X]] | + | [[Category: Xu, X]] |
| + | [[Category: Zhang, X]] | ||
| + | [[Category: Biotin-dependent decarboxylase]] | ||
| + | [[Category: Decarboxylase sodium pump]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Sodium pump]] | ||
Revision as of 09:54, 12 August 2020
Cryo-EM structure of the S. typhimurium oxaloacetate decarboxylase beta-gamma sub-complex
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