6m77
From Proteopedia
(Difference between revisions)
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==GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine== | ==GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine== | ||
| - | <StructureSection load='6m77' size='340' side='right'caption='[[6m77]]' scene=''> | + | <StructureSection load='6m77' size='340' side='right'caption='[[6m77]], [[Resolution|resolution]] 1.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M77 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M77 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m77]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis_tx4248 Enterococcus faecalis tx4248]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M77 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M77 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m77 OCA], [http://pdbe.org/6m77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m77 RCSB], [http://www.ebi.ac.uk/pdbsum/6m77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m77 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HMPREF9498_01557 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=749495 Enterococcus faecalis TX4248])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m77 OCA], [http://pdbe.org/6m77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m77 RCSB], [http://www.ebi.ac.uk/pdbsum/6m77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m77 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glycoside hydrolases catalyze the hydrolysis of glycosidic linkages in carbohydrates. The glycoside hydrolase family 31 (GH31) contains alpha-glucosidase, alpha-xylosidase, alpha-galactosidase, and alpha-transglycosylase. Recent work has expanded the diversity of substrate specificity of GH31 enzymes, and alpha-N-acetylgalactosaminidases (alphaGalNAcases) belonging to GH31 have been identified in human gut bacteria. Here, we determined the first crystal structure of a truncated form of GH31 alphaGalNAcase from the human gut bacterium Enterococcus faecalis. The enzyme has a similar fold to other reported GH31 enzymes and an additional fibronectin type 3-like domain. Additionally, the structure in complex with N-acetylgalactosamine reveals that conformations of the active site residues, including its catalytic nucleophile, change to recognize the ligand. Our structural analysis provides insight into the substrate recognition and catalytic mechanism of GH31 alphaGalNAcases. | ||
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| + | Crystal structure of the Enterococcus faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31.,Miyazaki T, Park EY FEBS Lett. 2020 May 4. doi: 10.1002/1873-3468.13804. PMID:32367553<ref>PMID:32367553</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6m77" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Enterococcus faecalis tx4248]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Miyazaki T]] | + | [[Category: Miyazaki, T]] |
| + | [[Category: Fibronectin-like]] | ||
| + | [[Category: Glycoside hydrolase]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Mucin]] | ||
Revision as of 09:56, 12 August 2020
GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine
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