6til
From Proteopedia
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==Structure of A. niger Fdc WT in complex with FMN and 2 naphthoic acid== | ==Structure of A. niger Fdc WT in complex with FMN and 2 naphthoic acid== | ||
| - | <StructureSection load='6til' size='340' side='right'caption='[[6til]]' scene=''> | + | <StructureSection load='6til' size='340' side='right'caption='[[6til]], [[Resolution|resolution]] 1.42Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TIL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6til]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/A._niger A. niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TIL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6til FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6til OCA], [http://pdbe.org/6til PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6til RCSB], [http://www.ebi.ac.uk/pdbsum/6til PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6til ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FIV:NAPHTHALENE-2-CARBOXYLIC+ACID'>FIV</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fdc1, An03g06590 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenacrylate_decarboxylase Phenacrylate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.102 4.1.1.102] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6til FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6til OCA], [http://pdbe.org/6til PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6til RCSB], [http://www.ebi.ac.uk/pdbsum/6til PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6til ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/FDC1_ASPNC FDC1_ASPNC]] Catalyzes the reversible decarboxylation of aromatic carboxylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives 4-vinylphenol, 4-vinylguaiacol, and styrene, respectively, which play the role of aroma metabolites.[HAMAP-Rule:MF_03196]<ref>PMID:26083754</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The direct C-H carboxylation of aromatic compounds is an attractive route to the corresponding carboxylic acids, but remains challenging under mild conditions. It has been proposed that the first step in anaerobic microbial degradation of recalcitrant aromatic compounds is a UbiD-mediated carboxylation. In this study, we use the UbiD enzyme ferulic acid decarboxylase (Fdc) in combination with a carboxylic acid reductase to create aromatic degradation-inspired cascade reactions, leading to efficient functionalization of styrene through CO2 fixation. We reveal that rational structure-guided laboratory evolution can expand the substrate scope of Fdc, resulting in activity on a range of mono- and bicyclic aromatic compounds through a single mutation. Selected variants demonstrated 150-fold improvement in the conversion of coumarillic acid to benzofuran + CO2 and unlocked reactivity towards naphthoic acid. Our data demonstrate that UbiD-mediated C-H activation is a versatile tool for the transformation of aryl/alkene compounds and CO2 into commodity chemicals. | ||
| + | |||
| + | Enzymatic C-H activation of aromatic compounds through CO2 fixation.,Aleku GA, Saaret A, Bradshaw-Allen RT, Derrington SR, Titchiner GR, Gostimskaya I, Gahloth D, Parker DA, Hay S, Leys D Nat Chem Biol. 2020 Jul 27. pii: 10.1038/s41589-020-0603-0. doi:, 10.1038/s41589-020-0603-0. PMID:32719558<ref>PMID:32719558</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6til" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: A. niger]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Leys D]] | + | [[Category: Phenacrylate decarboxylase]] |
| + | [[Category: Leys, D]] | ||
| + | [[Category: Aromatic acid]] | ||
| + | [[Category: Ligase]] | ||
| + | [[Category: Prfmn]] | ||
| + | [[Category: Ubid]] | ||
Revision as of 10:01, 12 August 2020
Structure of A. niger Fdc WT in complex with FMN and 2 naphthoic acid
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