5lsb

From Proteopedia

(Difference between revisions)

Revision as of 10:48, 12 August 2020

Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.

Structural highlights

5lsb is a 6 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	HSH49, YOR319W, O6142 (Baker's yeast), CUS1, YMR240C, YM9408.02C (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HSH49_YEAST] Possible SF3b-like factor. [CUS1_YEAST] Essential splicing protein required for U2 snRNP binding to pre-mRNA during spliceosome assembly.

Publication Abstract from PubMed

Spliceosomal proteins Hsh49p and Cus1p are components of SF3b, which together with SF3a, Msl1p/Lea1p, Sm proteins and U2 snRNA, form U2 snRNP, which plays a crucial role in pre-mRNA splicing. Hsh49p, comprising two RRMs, forms a heterodimer with Cus1p. We determined the crystal structures of Saccharomyces cerevisiae full-length Hsh49p as well as its RRM1 in complex with a minimal binding region of Cus1p (residues 290-368). The structures show that the Cus1 fragment binds to the alpha-helical surface of Hsh49p RRM1, opposite the four-stranded beta-sheet, leaving the canonical RNA binding surface available to bind RNA. Hsh49p binds the 5' end region of U2 snRNA via RRM1. Its affinity is increased in complex with Cus1(290-368)p, partly because an extended RNA-binding surface forms across the protein-protein interface. The Hsh49p RRM1-Cus1(290-368)p structure fits well into cryo-EM density of the Bact spliceosome, corroborating the biological relevance of our crystal structure.

Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p binding domain.,van Roon AA, Oubridge C, Obayashi E, Sposito B, Newman AJ, Seraphin B, Nagai K RNA. 2017 Mar 27. pii: rna.059378.116. doi: 10.1261/rna.059378.116. PMID:28348170^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ van Roon AA, Oubridge C, Obayashi E, Sposito B, Newman AJ, Seraphin B, Nagai K. Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p binding domain. RNA. 2017 Mar 27. pii: rna.059378.116. doi: 10.1261/rna.059378.116. PMID:28348170 doi:http://dx.doi.org/10.1261/rna.059378.116

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lsb"

@@ Line 1: / Line 1: @@
 ==Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.==
-<StructureSection load='5lsb' size='340' side='right' caption='[[5lsb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='5lsb' size='340' side='right'caption='[[5lsb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lsb]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LSB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lsb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LSB FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsb OCA], [http://pdbe.org/5lsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsb RCSB], [http://www.ebi.ac.uk/pdbsum/5lsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsb ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HSH49, YOR319W, O6142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), CUS1, YMR240C, YM9408.02C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsb OCA], [http://pdbe.org/5lsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsb RCSB], [http://www.ebi.ac.uk/pdbsum/5lsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsb ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Nagai, K]]
 [[Category: Obayashi, E]]

5lsb

From Proteopedia

Revision as of 10:48, 12 August 2020

Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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