5lxr

From Proteopedia

(Difference between revisions)

Revision as of 10:54, 12 August 2020

Structure of the minimal RBM7 - ZCCHC8 Complex

Structural highlights

5lxr is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Gene:	RBM7 (HUMAN), ZCCHC8 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RBM7_HUMAN] Possible involved in germ cell RNA processing and meiosis. [ZCHC8_HUMAN] May be involved in pre-mRNA splicing.

Publication Abstract from PubMed

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as the interaction site for the RNA-recognition motif (RRM) of RBM7 and present the crystal structure of the corresponding complex at 2.0 A resolution. On the basis of the structure, we identify a proline-rich segment within the splicing factor SAP145 with strong similarity to ZCCHC8. We show that this segment of SAP145 not only binds the RRM region of another splicing factor SAP49 but also the RRM of RBM7. These dual interactions of RBM7 with the exosome and the spliceosome suggest a model whereby NEXT might recruit the exosome to degrade intronic RNAs.

Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.,Falk S, Finogenova K, Melko M, Benda C, Lykke-Andersen S, Jensen TH, Conti E Nat Commun. 2016 Dec 1;7:13573. doi: 10.1038/ncomms13573. PMID:27905398^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Falk S, Finogenova K, Melko M, Benda C, Lykke-Andersen S, Jensen TH, Conti E. Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nat Commun. 2016 Dec 1;7:13573. doi: 10.1038/ncomms13573. PMID:27905398 doi:http://dx.doi.org/10.1038/ncomms13573

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lxr"

@@ Line 1: / Line 1: @@
 ==Structure of the minimal RBM7 - ZCCHC8 Complex==
-<StructureSection load='5lxr' size='340' side='right' caption='[[5lxr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='5lxr' size='340' side='right'caption='[[5lxr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lxr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LXR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lxr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LXR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxr OCA], [http://pdbe.org/5lxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxr RCSB], [http://www.ebi.ac.uk/pdbsum/5lxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxr ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RBM7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ZCCHC8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxr OCA], [http://pdbe.org/5lxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxr RCSB], [http://www.ebi.ac.uk/pdbsum/5lxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxr ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Benda, C]]
 [[Category: Conti, E]]

5lxr

From Proteopedia

Revision as of 10:54, 12 August 2020

Structure of the minimal RBM7 - ZCCHC8 Complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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