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5m0j

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Revision as of 10:57, 12 August 2020

Crystal structure of the cytoplasmic complex with She2p, She3p, and the ASH1 mRNA E3-localization element

Structural highlights

5m0j is a 10 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	5m0h, 5m0i
Gene:	SHE3, SCRG_02838 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SHE2_YEAS1] RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruits also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA (By similarity).

Publication Abstract from PubMed

mRNA localization is an essential mechanism of gene regulation and is required for processes such as stem-cell division, embryogenesis and neuronal plasticity. It is not known which features in the cis-acting mRNA localization elements (LEs) are specifically recognized by motor-containing transport complexes. To the best of our knowledge, no high-resolution structure is available for any LE in complex with its cognate protein complex. Using X-ray crystallography and complementary techniques, we carried out a detailed assessment of an LE of the ASH1 mRNA from yeast, its complex with its shuttling RNA-binding protein She2p, and its highly specific, cytoplasmic complex with She3p. Although the RNA alone formed a flexible stem loop, She2p binding induced marked conformational changes. However, only joining by the unstructured She3p resulted in specific RNA recognition. The notable RNA rearrangements and joint action of a globular and an unfolded RNA-binding protein offer unprecedented insights into the step-wise maturation of an mRNA-transport complex.

Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.,Edelmann FT, Schlundt A, Heym RG, Jenner A, Niedner-Boblenz A, Syed MI, Paillart JC, Stehle R, Janowski R, Sattler M, Jansen RP, Niessing D Nat Struct Mol Biol. 2017 Jan 16. doi: 10.1038/nsmb.3351. PMID:28092367^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Edelmann FT, Schlundt A, Heym RG, Jenner A, Niedner-Boblenz A, Syed MI, Paillart JC, Stehle R, Janowski R, Sattler M, Jansen RP, Niessing D. Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex. Nat Struct Mol Biol. 2017 Jan 16. doi: 10.1038/nsmb.3351. PMID:28092367 doi:http://dx.doi.org/10.1038/nsmb.3351

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5m0j"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the cytoplasmic complex with She2p, She3p, and the ASH1 mRNA E3-localization element==
-<StructureSection load='5m0j' size='340' side='right' caption='[[5m0j]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='5m0j' size='340' side='right'caption='[[5m0j]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5m0j]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M0J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5m0j]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M0J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M0J FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5m0h|5m0h]], [[5m0i|5m0i]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m0j OCA], [http://pdbe.org/5m0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m0j RCSB], [http://www.ebi.ac.uk/pdbsum/5m0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m0j ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SHE3, SCRG_02838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m0j OCA], [http://pdbe.org/5m0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m0j RCSB], [http://www.ebi.ac.uk/pdbsum/5m0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m0j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Edelmann, F T]]
 [[Category: Janowski, R]]

5m0j

From Proteopedia

Revision as of 10:57, 12 August 2020

Crystal structure of the cytoplasmic complex with She2p, She3p, and the ASH1 mRNA E3-localization element

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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